Difference between revisions of "Part:BBa K627007"

Latest revision as of 02:12, 22 September 2011

Fusion of c-myc-tag and gene III

The gene III protein is a coat protein from the filamentous bacteriophage M13 (Smith, 1985). It appears only 3-5 times on the tip of the phage and is responsible for infection of bacterial cells. It is usually fused to the gene of interest to determine protein-protein-interactions of its expression product using M13 phage display system. The gene III-protein consists of three domains: one C-termial and two N-terminal domains. Gene III was generated as biobrick several times for instance M13003 (2006) and M31537 (2007). It has been shown that proteins of interest fused to the carboxy-terminus of gene-III-proteins can be functionally displayed on the surface of phages (Fuh and Sidhu, 2000, Rakonjac et al., 1999). In the course of our project a phagemid was constructed carrying truncated gene III (only encoding C-terminal domain) as biobrick. To enable easy purification and detection of gene-III-fused proteins additionally a myc-tag-sequence was merged before gene –III-sequence.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K627007 short</partinfo>
-The gene III protein is a coat protein from the filamentous bacteriophage M13. It appears only 3-5 times on the tip of the phage and is responsible for infection of bacterial cells. It is usually fused to the gene of interest to determine protein-protein-interactions of its expression product using M13 phage display system. The gene III-protein consists of three domains: one C-termial and two N-terminal domains ( Holliger and Riechmann, 1997).
+The gene III protein is a coat protein from the filamentous bacteriophage M13 (Smith, 1985). It appears only 3-5 times on the tip of the phage and is responsible for infection of bacterial cells. It is usually fused to the gene of interest to determine protein-protein-interactions of its expression product using M13 phage display system. The gene III-protein consists of three domains: one C-termial and two N-terminal domains.
-Gene III was generated as biobrick several times for instance M13003 (2006) and M31537 (2007). It has been shown that proteins of interest fused to the carboxy-terminus of gene-III-proteins can be functionally displayed on the surface of phages (Fuh, 2000 and  Rakonjak, 1999). In the course of our project a phagemid was constructed carrying truncated geneIII (only C-terminus) as biobrick. To enable easy purification and detection of gene-III-fused proteins additionally a myc-tag-sequence was merged before gene –III-sequence.
+Gene III was generated as biobrick several times for instance M13003 (2006) and M31537 (2007). It has been shown that proteins of interest fused to the carboxy-terminus of gene-III-proteins can be functionally displayed on the surface of phages (Fuh and Sidhu, 2000, Rakonjac et al., 1999). In the course of our project a phagemid was constructed carrying truncated gene III (only encoding C-terminal domain) as biobrick. To enable easy purification and detection of gene-III-fused proteins additionally a myc-tag-sequence was merged before gene –III-sequence.