Difference between revisions of "Part:BBa K528021"
 
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===Construct design===
 
===Construct design===
  
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To make truly standardize strength of RBS parts we propose a brand new part in synthetic biology - we call expression adapters. They are short sequences consisting of RBS, 5 bp spacer and a short (10 amino acid long) beginning of a protein.
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This device was used to measure expression level obtained from <partinfo>K528018</partinfo> fused with Yellow Fluorescent Protein <partinfo>E0030</partinfo>.
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We have measured fluorescence level and compared it to <partinfo>K528010</partinfo> B0034+YFP reference construct.
  
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For more info please look at Team Warsaw 2011 http://2011.igem.org/Team:Warsaw/ExpressionAdaptors/Solution
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We have obtained the following results:
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<table class="tableizer-table">
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<tr class="tableizer-firstrow"><th>Part Name</th><th>Description</th><th>Expression level as %B0034+YFP</th><th>Std Dev.</th></tr> <tr><td>BBa_K528021</td><td>BBa_K528018+YFP measurement device</td><td>20,37%</td><td>3,21%</td></tr> <tr><td>reference</td><td>Bba_J23102+B0034+YFP (100% reference construct)</td><td>100,00%</td><td>2,61%</td></tr></table></html>
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According to design <partinfo>K528018</partinfo> has strength 22,86% relative to BBa_B0034 with given protein.
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This means that our expression adaptor works according to the design.
  
 
===Authors===
 
===Authors===

Latest revision as of 23:25, 21 September 2011

Expression Adapter fuse with E0030 YFP protein

Construct design

This device was used to measure expression level obtained from BBa_K528018 fused with Yellow Fluorescent Protein BBa_E0030. We have measured fluorescence level and compared it to BBa_K528010 B0034+YFP reference construct.

We have obtained the following results:

Part NameDescriptionExpression level as %B0034+YFPStd Dev.
BBa_K528021BBa_K528018+YFP measurement device20,37%3,21%
referenceBba_J23102+B0034+YFP (100% reference construct)100,00%2,61%


According to design BBa_K528018 has strength 22,86% relative to BBa_B0034 with given protein. This means that our expression adaptor works according to the design.

Authors

Clonning:

  • Paweł Urbański
  • Elżbieta Jankowska
  • Krzysztof Szczepaniak
  • Dorota Kaja Sabat

The lab work was supervised by:

  • Michał Lower
  • Ania Olchowik


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 8
    Illegal NheI site found at 31
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]