Difference between revisions of "Part:BBa K525005:Design"
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* <partinfo>K525301</partinfo> S-layer gene ''sgsE'' synthesized, originated in ''Geobacillus stearothermophilus'' NRS 2004/3a | * <partinfo>K525301</partinfo> S-layer gene ''sgsE'' synthesized, originated in ''Geobacillus stearothermophilus'' NRS 2004/3a | ||
− | * <partinfo> | + | * <partinfo>K525720</partinfo> NAD<sup>+</sup> dependent DNA ligase from ''Escherichia coli'' TOP10 |
Latest revision as of 19:09, 21 September 2011
Fusion Protein of S-Layer SgsE and LigA
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 167
Illegal BglII site found at 1022
Illegal BglII site found at 3819 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 76
Illegal AgeI site found at 4417 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1657
Illegal SapI.rc site found at 4033
Design Notes
Assembled via Freiburg BioBrick assembly standard (RFC 25) from BBa_K525303 and BBa_K525720
Source
- BBa_K525303 synthesized, codon optimized and in Freiburg assembly standard (RFC 25) to easily create fusion proteins
- Promoter: fusion promoter between T7 promoter and lac-operator
- T7 promoter from T7 phage
- lac-operator from E. coli
- BBa_K525301 S-layer gene sgsE synthesized, originated in Geobacillus stearothermophilus NRS 2004/3a
- BBa_K525720 NAD+ dependent DNA ligase from Escherichia coli TOP10
References
Kainz B, Steiner K, Möller M, Pum D, Schäffer C, Sleytr UB, Toca-Herrera JL (2010) Absorption, Steady-State Fluorescence, Fluorescence Lifetime, and 2D Self-Assembly Properties of Engineered Fluorescent S-Layer Fusion Proteins of Geobacillus stearothermophilus NRS 2004/3a, [http://pubs.acs.org/doi/abs/10.1021/bm901071b Biomacromolecules 11(1):207-214].
Sleytr UB, Huber C, Ilk N, Pum D, Schuster B, Egelseer EM (2007) S-layers as a tool kit for nanobiotechnological applications, [http://onlinelibrary.wiley.com/doi/10.1111/j.1574-6968.2006.00573.x/full FEMS Microbiol Lett 267(2):131-144].
Tang Z, Liu P, Ma C, Yang X, Wang K, Tan W, Lv X (2011) Molecular Beacon Based Bioassay for Highly Sensitive and Selective Detection of Nicotinamide Adenine Dinucleotide and the Activity of Alanine Aminotransferase, [http://pubs.acs.org/doi/abs/10.1021/ac102742k Anal Chem 83(7):2505-2510].