Difference between revisions of "Part:BBa K568005:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | This part was constructed in order to add T7ptag (<html><a href="https://parts.igem.org Part:BBa_K228000">BBa_K228000</a></html> at 3' site of intermediate construct in a next step. | + | This part was constructed in order to add T7ptag (<html><a href="https://parts.igem.org Part:BBa_K228000">BBa_K228000</a></html>) at 3' site of intermediate construct in a next step. |
===Source=== | ===Source=== | ||
Latest revision as of 00:13, 22 September 2011
Intermediate optogenetical AND-Gate without T7ptag
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 644
Illegal XhoI site found at 1786 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 971
Illegal AgeI site found at 3817 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 3820
Design Notes
This part was constructed in order to add T7ptag (BBa_K228000) at 3' site of intermediate construct in a next step.
Source
The red light sensor (BBa_K568000) was added to an intermediated part of the optogenetic AND gate (BBa_K568006).