Difference between revisions of "Part:BBa K607010"
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<partinfo>BBa_K607010 short</partinfo> | <partinfo>BBa_K607010 short</partinfo> | ||
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| + | This part is a member of [https://parts.igem.org/Lambda_cI_autoinducing_loop_family Lambda cI autoinducing loop family]. | ||
cI with LVA-tag under transcriptional control of Prm promoter and reverse antisense lasB promoter, | cI with LVA-tag under transcriptional control of Prm promoter and reverse antisense lasB promoter, | ||
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This construct represents an auto-inducing loop in which the Prm promoter drives expression of its own inducer cI. The running loop can be turned off by activating the reverse antisense lasB promoter (through LasR protein in the presence of Pseudomonas autoinducer (PAI)) | This construct represents an auto-inducing loop in which the Prm promoter drives expression of its own inducer cI. The running loop can be turned off by activating the reverse antisense lasB promoter (through LasR protein in the presence of Pseudomonas autoinducer (PAI)) | ||
| − | [[Image:lciau1.png | + | |
| − | [[Image:lciaulegend.png]] | + | [[Image:lciau1.png|Caption test]] |
| + | [[Image:lciaulegend.png|frameless]] | ||
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| + | '''Figure 1.''' This autoinducing loop does not exhibit heterogenity when non-induced. Only the population that is not producing GFP is observed. That means that the loop is not induced through a leaky promoter and is a good candidate for an inducible bistable switch. | ||
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===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K607010 parameters</partinfo> | <partinfo>BBa_K607010 parameters</partinfo> | ||
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Latest revision as of 16:28, 21 September 2011
Prm_cI-LVA_antiPlasB with 0.07RBS
This part is a member of Lambda cI autoinducing loop family.
cI with LVA-tag under transcriptional control of Prm promoter and reverse antisense lasB promoter, the RBS has a strength of 0.07 (obtained by Jason Kelly and Robbie Bryant during the summer of 2004 using a fluorescent reporter). This construct represents an auto-inducing loop in which the Prm promoter drives expression of its own inducer cI. The running loop can be turned off by activating the reverse antisense lasB promoter (through LasR protein in the presence of Pseudomonas autoinducer (PAI))
Figure 1. This autoinducing loop does not exhibit heterogenity when non-induced. Only the population that is not producing GFP is observed. That means that the loop is not induced through a leaky promoter and is a good candidate for an inducible bistable switch.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1125
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]