Difference between revisions of "Part:BBa K515107:Design"
 
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===Design Notes===
 
===Design Notes===
 
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<p>The original Dendra2 sequence had been joint codon optimized for <i>Escherichia coli</i> and <i>Bacillus subtilis<i> and designed to contain the insulator sequence under the influence of a strong RBS. However it could not be synthesised. We therefore allowed Eurofins to codon optimize it for <i>E. coli</i> and only had the coding region synthesised with a prefix and a suffix. Once the gene arrived, we assembled it using standard BioBrick assembly with the TetR repressible promoter <a href="https://parts.igem.org/Part:BBa_R0040"/>(BBa_R0040)</a> and a standard RBS <a href="https://parts.igem.org/Part:BBa_R0034"/>(BBa_R0034)</a>.
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<p>The original Dendra2 sequence had been joint codon optimized for <i>Escherichia coli</i> and <i>Bacillus subtilis</i> and designed to contain the insulator sequence under the influence of a strong RBS. However it could not be synthesised. We therefore allowed Eurofins to codon optimize it for <i>E. coli</i> and only had the coding region synthesised with a prefix and a suffix. Once the gene arrived, we assembled it using standard BioBrick assembly with the TetR repressible promoter <a href="https://parts.igem.org/Part:BBa_R0040"/>(BBa_R0040)</a> and a standard RBS <a href="https://parts.igem.org/Part:BBa_R0034"/>(BBa_R0034)</a>.
  
 
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Latest revision as of 14:37, 21 September 2011

Composite p(tetR) - Dendra2


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The original Dendra2 sequence had been joint codon optimized for Escherichia coli and Bacillus subtilis and designed to contain the insulator sequence under the influence of a strong RBS. However it could not be synthesised. We therefore allowed Eurofins to codon optimize it for E. coli and only had the coding region synthesised with a prefix and a suffix. Once the gene arrived, we assembled it using standard BioBrick assembly with the TetR repressible promoter (BBa_R0040) and a standard RBS (BBa_R0034).

Source

-

References