Difference between revisions of "Part:BBa K551000"
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<partinfo>BBa_K551000 short</partinfo> | <partinfo>BBa_K551000 short</partinfo> | ||
| − | This part is composed of two mutated Flipase Recognition Targets (FRT') flanking the Chloramphenicol resistance gene. The mutation removes the | + | This part is composed of two mutated Flipase Recognition Targets (FRT') flanking the Chloramphenicol resistance gene. The mutation removes the XbaI restriction site from the canonical FRT sequence. It is similar to BBa_J61025 but without the scar between the first FRT' and CmR. |
The two FRT' sequences allow the excision of the flanked sequence by the Flipase recombinase. | The two FRT' sequences allow the excision of the flanked sequence by the Flipase recombinase. | ||
Latest revision as of 16:07, 21 September 2011
FRT-CM-FRT
This part is composed of two mutated Flipase Recognition Targets (FRT') flanking the Chloramphenicol resistance gene. The mutation removes the XbaI restriction site from the canonical FRT sequence. It is similar to BBa_J61025 but without the scar between the first FRT' and CmR.
The two FRT' sequences allow the excision of the flanked sequence by the Flipase recombinase.
It's been tested with success for resistance to Chloramphenicol and excision per the pINDEL plasmid.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]