Difference between revisions of "Part:BBa K564012"
 
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ChiP encodes enterobacterial chitoporin required for uptake of chitin-derived oligosaccharides. This outer membrane porin is required for Salmonella and E.coli growth on chitooligosaccharides as a sole source of both carbon and nitrogen. It is only synthesized in the presence of these substrates. In nature it is regulated by sRNAs.The 5' untranslated region and promoter of this gene is fused with reporter gene lacZ and is donwlregulated by sRNA chiX (also known as micM or sroB).
 
ChiP encodes enterobacterial chitoporin required for uptake of chitin-derived oligosaccharides. This outer membrane porin is required for Salmonella and E.coli growth on chitooligosaccharides as a sole source of both carbon and nitrogen. It is only synthesized in the presence of these substrates. In nature it is regulated by sRNAs.The 5' untranslated region and promoter of this gene is fused with reporter gene lacZ and is donwlregulated by sRNA chiX (also known as micM or sroB).
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This was verified by β-gal assays as shown in the table below. The measurements are in Miller Units
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{| class="wikitable"
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|-
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! Strain
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! [http://ecoliwiki.net/colipedia/index.php/NM522 NM522]
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! [http://2011.igem.org/Team:DTU-Denmark/Project_testing_sRNA#Strain_construction IG302]
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|-
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| Genotype
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| chiX +
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| chiX -
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|-
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| [https://parts.igem.org/wiki/index.php?title=Part:BBa_K564012 '''PchiP(original):lacZ, BBa_K564012''']
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| 467
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| 4781
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|-
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| [https://parts.igem.org/wiki/index.php?title=Part:BBa_K564013 '''PchiP(mutated):lacZ, BBa_K564013''']
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| 2353
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| 2403
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|}
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 +
This system will not work in a strain deficient in hfq.
  
 
<!-- Add more about the biology of this part here
 
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Latest revision as of 01:51, 22 September 2011

promoter and RBS of E. coli chitoporin translationally fused with lacZ.

ChiP encodes enterobacterial chitoporin required for uptake of chitin-derived oligosaccharides. This outer membrane porin is required for Salmonella and E.coli growth on chitooligosaccharides as a sole source of both carbon and nitrogen. It is only synthesized in the presence of these substrates. In nature it is regulated by sRNAs.The 5' untranslated region and promoter of this gene is fused with reporter gene lacZ and is donwlregulated by sRNA chiX (also known as micM or sroB).

This was verified by β-gal assays as shown in the table below. The measurements are in Miller Units

Strain [http://ecoliwiki.net/colipedia/index.php/NM522 NM522] [http://2011.igem.org/Team:DTU-Denmark/Project_testing_sRNA#Strain_construction IG302]
Genotype chiX + chiX -
PchiP(original):lacZ, BBa_K564012 467 4781
PchiP(mutated):lacZ, BBa_K564013 2353 2403

This system will not work in a strain deficient in hfq.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]