Difference between revisions of "Part:BBa K613010"
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Parts K613007 through K613012 are T7 promoter variants containing the lac operator just downstream of the T7 promoter. K613007 contains the T7 promoter wildtype sequence. The other sequences were derived by single base pair mutations that have been shown to alter its strength. The part includes a strong ribosomal binding site. Its strength was determined by cloning it in front of mRFP1 reporter and measuring fluorescence of BL21 cells containing a low copy number plasmid containing the reporter construct. | Parts K613007 through K613012 are T7 promoter variants containing the lac operator just downstream of the T7 promoter. K613007 contains the T7 promoter wildtype sequence. The other sequences were derived by single base pair mutations that have been shown to alter its strength. The part includes a strong ribosomal binding site. Its strength was determined by cloning it in front of mRFP1 reporter and measuring fluorescence of BL21 cells containing a low copy number plasmid containing the reporter construct. | ||
| − | + | See also parts K613001 through K613006 for the same T7 promoter variants without a lac operator. | |
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| + | [[Image:T7variants.png|700px]] | ||
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| + | For more characterization data see our [http://2011.igem.org/Team:EPF-Lausanne/Our_Project/T7_promoter_variants/t7prom wiki]. | ||
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| + | 1. Imburgio, D., Rong, M., Ma, K. & McAllister, W.T. Studies of promoter recognition and start site selection by T7 RNA polymerase using a comprehensive collection of promoter variants. Biochemistry 39, 10419–10430 (2000). | ||
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| + | <!-- Add more about the biology of this part here | ||
| + | <!-- --> | ||
| + | <span class='h3bb'>Usage and Biology</span> | ||
| + | <p> ITB_Indonesia 2015 has used this promoter to express their rhlAB enzymes </p> | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
Latest revision as of 09:28, 18 September 2015
T7 promoter family member (LacI repressed)
Parts K613007 through K613012 are T7 promoter variants containing the lac operator just downstream of the T7 promoter. K613007 contains the T7 promoter wildtype sequence. The other sequences were derived by single base pair mutations that have been shown to alter its strength. The part includes a strong ribosomal binding site. Its strength was determined by cloning it in front of mRFP1 reporter and measuring fluorescence of BL21 cells containing a low copy number plasmid containing the reporter construct.
See also parts K613001 through K613006 for the same T7 promoter variants without a lac operator.
For more characterization data see our [http://2011.igem.org/Team:EPF-Lausanne/Our_Project/T7_promoter_variants/t7prom wiki].
1. Imburgio, D., Rong, M., Ma, K. & McAllister, W.T. Studies of promoter recognition and start site selection by T7 RNA polymerase using a comprehensive collection of promoter variants. Biochemistry 39, 10419–10430 (2000).
Usage and Biology
ITB_Indonesia 2015 has used this promoter to express their rhlAB enzymes
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]