Difference between revisions of "Part:BBa K584021"
 
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<p align="justify">To know more about the inaZ, it is suggested to read this [http://www.ncbi.nlm.nih.gov/pubmed/10933828 article] describing the usage of pUC1813ICE with the '''inaZ'''.</p>
 
<p align="justify">To know more about the inaZ, it is suggested to read this [http://www.ncbi.nlm.nih.gov/pubmed/10933828 article] describing the usage of pUC1813ICE with the '''inaZ'''.</p>
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The UGent Belgium 2016 iGEM team improved this part by removing 2 BsaI restriction sites in order to make it compatible with Golden Gate assembly/MoClo, creating [[Part:BBa_K1896011|BBa_K1896011]].
  
 
<!-- Add more about the biology of this part here
 
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Latest revision as of 15:08, 19 October 2016

Ice Nucleating Protein Gene - inaZ

This part contains the Ice Nucleating Protein coding sequence. The ice nucleation protein (INP) is a glycosyl phosphatidylinositol anchored outer membrane protein found in certain Gram-negative bacteria. inaZ is the gene responsible for producing the ice nucleation protein.

We would like to thank [http://www.academicbiography.uwo.ca/profile.php?&n=ggloor Prof. Dr. Gregory Gloor ], for providing us with a plasmid (pUC1813ICE) which contains the ice nucleating protein gene inaZ.

To know more about the inaZ, it is suggested to read this [http://www.ncbi.nlm.nih.gov/pubmed/10933828 article] describing the usage of pUC1813ICE with the inaZ.

The UGent Belgium 2016 iGEM team improved this part by removing 2 BsaI restriction sites in order to make it compatible with Golden Gate assembly/MoClo, creating BBa_K1896011.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 646
    Illegal NgoMIV site found at 2158
    Illegal NgoMIV site found at 2302
    Illegal NgoMIV site found at 2566
    Illegal NgoMIV site found at 2710
    Illegal NgoMIV site found at 2758
    Illegal NgoMIV site found at 2878
    Illegal AgeI site found at 417
    Illegal AgeI site found at 1822
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 592
    Illegal BsaI.rc site found at 3338
    Illegal SapI.rc site found at 238