Difference between revisions of "Part:BBa K539711:Design"

 
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<partinfo>BBa_K539711 short</partinfo>
 
<partinfo>BBa_K539711 short</partinfo>
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===Design Notes===
 
===Design Notes===
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Because of the four RE site, EcoR1, Xba1, Spe1 and Pst1, before and after the coding gene, we have to delete those RE site in kivd. The RE site in the part has been modified by point mutation.
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Because of the four RE site, EcoR1, Xba1, Spe1 and Pst1, before and after the coding region, those RE site should not exist in the coding region.There are two  EcoR1 site in original sequence of ilvC. Those RE site in the ilvC has been modified by point mutation.
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<br>https://static.igem.org/mediawiki/parts/0/0b/Ilvc.png
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===Source===
 
===Source===
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The gene is cloned by Zeng , NCTU-Formosa.
 
It comes from Escherichia coli str. K-12 substr. MG1655
 
It comes from Escherichia coli str. K-12 substr. MG1655
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===References===
 
===References===

Latest revision as of 10:44, 8 October 2011

ilvC (ketol-acid reductoisomerase NAD(P)-binding)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 592
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 178
    Illegal BsaI.rc site found at 997


Design Notes

Because of the four RE site, EcoR1, Xba1, Spe1 and Pst1, before and after the coding region, those RE site should not exist in the coding region.There are two EcoR1 site in original sequence of ilvC. Those RE site in the ilvC has been modified by point mutation.
Ilvc.png

Source

The gene is cloned by Zeng , NCTU-Formosa. It comes from Escherichia coli str. K-12 substr. MG1655

References