Difference between revisions of "Part:BBa K564014"

(New page: _NOTOC__ <partinfo>BBa_K564014 short</partinfo> This device is based on gene encoding small RNA (sRNA), which represses ChiP gene synthesis by pairing with a sequence in 5’ untranslated...)
 
 
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_NOTOC__
 
 
<partinfo>BBa_K564014 short</partinfo>
 
<partinfo>BBa_K564014 short</partinfo>
  
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This device is based on gene encoding small RNA (sRNA), which represses ChiP gene synthesis by pairing with a sequence in 5’ untranslated region (UTR) near ribosome binding site (RBS) of chiP mRNA. This leads to degradation of the targeted mRNA by enzymes called RNases.The repression occurs in the absence of the substrates (chitooligosaccharides) stimulating chiP gene transcription. It is fused with Ptet promoter.  
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This part contains a anhydrotetracycline regulatable promoter and the gene for chiX downstream of it. Upon aTC addition, it triggers expression of the sRNA chiX.
  
  

Latest revision as of 13:17, 21 September 2011

atC-->chiX

This part contains a anhydrotetracycline regulatable promoter and the gene for chiX downstream of it. Upon aTC addition, it triggers expression of the sRNA chiX.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 848
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 838
  • 1000
    COMPATIBLE WITH RFC[1000]