Difference between revisions of "Part:BBa K590017"
 
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<partinfo>BBa_K590017 short</partinfo>
 
<partinfo>BBa_K590017 short</partinfo>
  
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This part is made by [http://2011.igem.org/Team:Washington UW iGEM Team 2011]. It includes 6 genes, which were extracted into 4 manageable sized inserts [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590000 mamHI], [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590001 mamE], [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590002 mamJ], [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590003 mamKL] in a pGA3k3 plasmid backbone. All the genes were found essential for magnetosome formation in ''Magnetospirillum magneticum'' strain AMB-1 on a [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590014 pGA3k3 plasmid backbone].
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This part is made by [http://2011.igem.org/Team:Washington UW iGEM Team 2011]. It includes 6 genes [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590000 mamHI], [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590001 mamE], [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590002 mamJ], [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590003 mamKL] as well as the native <i>mamAB</i> promoter, all in a pGA3k3 plasmid backbone. All the genes were found essential for magnetosome formation in ''Magnetospirillum magneticum'' strain AMB-1.
  
 
===Usage and Biology===
 
===Usage and Biology===
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Made by Gibson Cloning method instead of Standard Biobrick method, this part is in low copy backbone and it belongs to the [http://2011.igem.org/Team:Washington/Magnetosomes/Magnet_Toolkit Magnetosome Toolkit], known as the "first part of the full assembly".
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We made this construct from 2 kilobase gene extractions from AMB-1. These fragments were assembled using the [http://2010.igem.org/Team:Cambridge/Gibson/RFC Gibson cloning] method instead of standard assembly. This super-assembly is known as "Part one" of the full 16kb <i>mamAB</i> reassembly project. This part is in a medium copy backbone and it belongs to the [http://2011.igem.org/Team:Washington/Magnetosomes/Magnet_Toolkit Magnetosome Toolkit].  
  
 
[[Image:IGEM11 HIEJKL partregistry.png|500px|center]]
 
[[Image:IGEM11 HIEJKL partregistry.png|500px|center]]
  
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Cell chain formation was observed in microscopy when this plasmid was transformed in ''E.coli''.
 
  
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[[Image:HIEJKL 1b wiki.png|500px|center]]
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After Gibson cloning and transformation, cell chain formation was observed in microscopy(below) when this plasmid was transformed in ''E.coli''.
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[[Image:HIEJKL 1b wiki.png|500px|thumb|center]]
  
  

Latest revision as of 23:18, 28 September 2011

mamHIEJKL in vector pGA3K3

This part is made by [http://2011.igem.org/Team:Washington UW iGEM Team 2011]. It includes 6 genes mamHI, mamE, mamJ, mamKL as well as the native mamAB promoter, all in a pGA3k3 plasmid backbone. All the genes were found essential for magnetosome formation in Magnetospirillum magneticum strain AMB-1.

Usage and Biology

We made this construct from 2 kilobase gene extractions from AMB-1. These fragments were assembled using the [http://2010.igem.org/Team:Cambridge/Gibson/RFC Gibson cloning] method instead of standard assembly. This super-assembly is known as "Part one" of the full 16kb mamAB reassembly project. This part is in a medium copy backbone and it belongs to the [http://2011.igem.org/Team:Washington/Magnetosomes/Magnet_Toolkit Magnetosome Toolkit].

IGEM11 HIEJKL partregistry.png











After Gibson cloning and transformation, cell chain formation was observed in microscopy(below) when this plasmid was transformed in E.coli.

HIEJKL 1b wiki.png


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 5175
    Illegal PstI site found at 8836
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 5175
    Illegal PstI site found at 8836
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 8540
    Illegal XhoI site found at 89
    Illegal XhoI site found at 6375
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 5175
    Illegal PstI site found at 8836
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 5175
    Illegal PstI site found at 8836
    Illegal NgoMIV site found at 238
    Illegal NgoMIV site found at 1254
    Illegal NgoMIV site found at 2589
    Illegal NgoMIV site found at 3920
    Illegal NgoMIV site found at 6754
    Illegal NgoMIV site found at 8562
    Illegal NgoMIV site found at 8929
    Illegal AgeI site found at 5849
    Illegal AgeI site found at 6264
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 651
    Illegal BsaI site found at 1986
    Illegal BsaI site found at 3317
    Illegal BsaI.rc site found at 6947
    Illegal SapI site found at 825
    Illegal SapI site found at 2160
    Illegal SapI site found at 3491
    Illegal SapI.rc site found at 9208
    Illegal SapI.rc site found at 9529