Difference between revisions of "Part:BBa K564011"

Latest revision as of 15:28, 21 September 2011

ara-->chiXR (sRNA) device

ChiXR originates from the integenic region between genes chbB and chbC of enterobacteriacea. chiXR is a poorly defined sRNA that regulates the chiP gene through trapping the downregulating chiX sRNA. chiXR therefore has an indirect stimulatory effect on ChiP synthesis. The E. coli chiXR gene has been put under the control of the arabinose promoter in this biobrick so as to easily control its expression.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1205
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1144
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 979
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 961

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 __NOTOC__
 <partinfo>BBa_K564011 short</partinfo>
-ChiXR originates from an intercistronic region of the chb operon in Salmonella and E. coli. The transcription of this operon is activated in the presence of the chitooligosaccharides as the genes included in this operon comprises for metabolism of these sugars. ChiXR destabilizes another regulatory small RNA called ChiX by binding to the stem of ChiX transcription terminator hairpin. It is fused with arabinose promoter (Para).
+ChiXR originates from the integenic region between genes ''chbB'' and ''chbC'' of enterobacteriacea. ''chiXR'' is a poorly defined sRNA that regulates the ''chiP'' gene through trapping the downregulating ''chiX'' sRNA. ''chiXR'' therefore has an indirect stimulatory effect on ChiP synthesis.
+The ''E. coli'' ''chiXR'' gene has been put under the control of the arabinose promoter in this biobrick so as to easily control its expression.
 <!-- Add more about the biology of this part here