Difference between revisions of "Part:BBa K590060"
 
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<partinfo>BBa_K590060 short</partinfo>
 
<partinfo>BBa_K590060 short</partinfo>
  
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This part is a synthesized version of the Lux D gene from the Cambridge 2010 LuxBrick
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This part consists of a synthesized version of the ''luxD'' gene from the [http://2010.igem.org/Team:Cambridge/Bioluminescence/Bacterial_Luciferases Cambridge 2010 LuxBrick]. It is optimized to have cloning-friendly GC content and is also codon-optimized for expression in ''Escherichia coli''.
  
 
===Usage and Biology===
 
===Usage and Biology===
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University of Washington 2011 iGEM Team has tried to use this part along with [https://parts.igem.org/Part:BBa_K590059 Lux C] and [https://parts.igem.org/Part:BBa_K590061 Lux E] to engineer alternative routes for fatty aldehyde production. For more information, please refer to the [http://2011.igem.org/Team:Washington/Alkanes/Future/LuxCDE Alternative Routes for Fatty Aldehyde Production] in University of Washington 2011 iGEM Team's wiki.
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The [http://2011.igem.org/Team:Washington University of Washington 2011 iGEM Team] has tried to use this part along with [https://parts.igem.org/Part:BBa_K590059 Lux C] and [https://parts.igem.org/Part:BBa_K590061 Lux E] to engineer alternative routes for fatty aldehyde production.[[Image:Washington_luxcde_diagram.png|400px|center]]
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[[Image:Washington luxcde diagram.png|400px]]
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Latest revision as of 05:02, 28 September 2011

LuxD; standalone, E. Coli codon optimized

This part consists of a synthesized version of the luxD gene from the [http://2010.igem.org/Team:Cambridge/Bioluminescence/Bacterial_Luciferases Cambridge 2010 LuxBrick]. It is optimized to have cloning-friendly GC content and is also codon-optimized for expression in Escherichia coli.

Usage and Biology

The [http://2011.igem.org/Team:Washington University of Washington 2011 iGEM Team] has tried to use this part along with Lux C and Lux E to engineer alternative routes for fatty aldehyde production.
Washington luxcde diagram.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 247
    Illegal BsaI site found at 616