Difference between revisions of "Part:BBa K678052"
 
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pJEJAM4 is a plasmid intended for transient transfection of mammalian cells. pJEJAM4 consists of the strong constitutive cytomegalovirus (CMV) promoter, mCherry, the ''BGK'' polyA, the ''hygR'' marker cassette, an ori for propagation in ''E.coli'' and, ampR resistance cassette (Figure 1).
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pJEJAM4 is a plasmid intended for transient transfection of mammalian cells. pJEJAM4 consists of the strong constitutive cytomegalovirus (CMV) promoter, mCherry, the BGH polyA, the ''hygR'' marker cassette, an ori for propagation in ''E.coli'', and an ampR resistance cassette (Figure 1).
  
 
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Expression of pJEJAM4 in the mammalian cell line U-2 OS results in a homogenous distribution of red fluorescent protein in the cells (Figure 2 and Figure 3).
  
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[[Image:5_rød-2.png|left|300px|thumb|<b>DTU-Denmark-2 2011</b> Figure 2: .]]
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[[Image:5_red-2.png|left|440px|thumb|<b>DTU-Denmark-2 2011</b> Figure 2: U-2 OS cells transiently transfected with pJEJAM4 expressing mCherry. The white bar has a length of 70μm.]]
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[[Image:26.08.png|right|300px|thumb|<b>DTU-Denmark-2 2011</b> Figure 3: .]]
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[[Image:26.08.png|right|440px|thumb|<b>DTU-Denmark-2 2011</b> Figure 3: U-2 OS cells transiently transfected with pJEJAM4 expressing mCherry. The white bar has a length of 50μm.]]
 
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Latest revision as of 15:24, 27 September 2011

pJEJAM4

pJEJAM4 is a plasmid intended for transient transfection of mammalian cells. pJEJAM4 consists of the strong constitutive cytomegalovirus (CMV) promoter, mCherry, the BGH polyA, the hygR marker cassette, an ori for propagation in E.coli, and an ampR resistance cassette (Figure 1).



DTU-Denmark-2 2011 Figure 1: Plasmid map of pJEJAM4, the figure is not drawn to scale.



Expression of pJEJAM4 in the mammalian cell line U-2 OS results in a homogenous distribution of red fluorescent protein in the cells (Figure 2 and Figure 3).

DTU-Denmark-2 2011 Figure 2: U-2 OS cells transiently transfected with pJEJAM4 expressing mCherry. The white bar has a length of 70μm.
DTU-Denmark-2 2011 Figure 3: U-2 OS cells transiently transfected with pJEJAM4 expressing mCherry. The white bar has a length of 50μm.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2207
    Illegal PstI site found at 1021
    Illegal PstI site found at 2297
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2207
    Illegal PstI site found at 1021
    Illegal PstI site found at 2297
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2207
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2207
    Illegal PstI site found at 1021
    Illegal PstI site found at 2297
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2207
    Illegal PstI site found at 1021
    Illegal PstI site found at 2297
    Illegal NgoMIV site found at 3062
  • 1000
    COMPATIBLE WITH RFC[1000]