Difference between revisions of "Part:BBa K590017:Experience"

Latest revision as of 07:33, 22 September 2011

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Please enter how you used this part and how it worked out.

Applications of BBa_K590017

User Reviews

We first extracted the individual genes as 4 inserts (down to a size that is more manageable), then we use Gibson Assembly method to put the inserts and the plasmid backbone together. The formation of cell chains can possibly be an indication of correct expression because the genes were reported to be important for chain alignments. However, this has to be further studied.

UNIQe92c7737b879aa34-partinfo-00000000-QINU UNIQe92c7737b879aa34-partinfo-00000001-QINU

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 ===User Reviews===
-We first extracted the individual genes(in a size that is more manageable), then we use Gibson Assembly method to put the genes and the plasmid backbone together.
+We first extracted the individual genes as 4 inserts (down to a size that is more manageable), then we use Gibson Assembly method to put the inserts and the plasmid backbone together. The formation of cell chains can possibly be an indication of correct expression because the genes were reported to be important for chain alignments. However, this has to be further studied.
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