Difference between revisions of "Part:BBa K678051"
 
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pJEJAM3 is a plasmid intended for transient transfection of mammalian cells. pJEJAM3 consists of the strong constitutive cytomegalovirus (CMV) promoter, yellow fluorescent protein (YFP), the ''BGK'' polyA, the ''hygR'' marker cassette, an ori for propagation in ''E.coli'' and, ampR resistance cassette (Figure 1).
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pJEJAM3 is a plasmid intended for transient transfection of mammalian cells. pJEJAM3 consists of the strong constitutive cytomegalovirus (CMV) promoter, yellow fluorescent protein (YFP), the BGH polyA, the ''hygR'' marker cassette, an ori for propagation in ''E.coli'', and an ampR resistance cassette (Figure 1).
  
 
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Confocal microscopy showed that expression of pJEJAM 3 in the mammalian cell line U-2 OS results in a homogenous distribution of YFP in the cells (Figure 2 and Figure 3).
  
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[[Image:14%2B16_gul.png|left|300px|thumb|<b>DTU-Denmark-2 2011</b> Figure 2: .]]
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[[Image:14%2B16_gul.png|left|440px|thumb|<b>DTU-Denmark-2 2011</b> Figure 2: U-2 OS cells transiently transfected with pJEJAM4 expressing YFP. The white bar has a length of 40μm.]]
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[[Image:1_14_gul.png|right|300px|thumb|<b>DTU-Denmark-2 2011</b> Figure 3: .]]
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[[Image:1_14_gul.png|right|440px|thumb|<b>DTU-Denmark-2 2011</b> Figure 3: U-2 OS cells transiently transfected with pJEJAM4 expressing YFP. The white bar has a length of 30μm.]]
 
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Latest revision as of 14:25, 27 September 2011

pJEJAM3

pJEJAM3 is a plasmid intended for transient transfection of mammalian cells. pJEJAM3 consists of the strong constitutive cytomegalovirus (CMV) promoter, yellow fluorescent protein (YFP), the BGH polyA, the hygR marker cassette, an ori for propagation in E.coli, and an ampR resistance cassette (Figure 1).



DTU-Denmark-2 2011 Figure 1: Plasmid map of pJEJAM3, the figure is not drawn to scale.



Confocal microscopy showed that expression of pJEJAM 3 in the mammalian cell line U-2 OS results in a homogenous distribution of YFP in the cells (Figure 2 and Figure 3).

DTU-Denmark-2 2011 Figure 2: U-2 OS cells transiently transfected with pJEJAM4 expressing YFP. The white bar has a length of 40μm.
DTU-Denmark-2 2011 Figure 3: U-2 OS cells transiently transfected with pJEJAM4 expressing YFP. The white bar has a length of 30μm.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1416
    Illegal EcoRI site found at 2294
    Illegal XbaI site found at 1459
    Illegal PstI site found at 874
    Illegal PstI site found at 1421
    Illegal PstI site found at 2384
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1416
    Illegal EcoRI site found at 2294
    Illegal PstI site found at 874
    Illegal PstI site found at 1421
    Illegal PstI site found at 2384
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1416
    Illegal EcoRI site found at 2294
    Illegal BglII site found at 1396
    Illegal BamHI site found at 1447
    Illegal XhoI site found at 1400
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1416
    Illegal EcoRI site found at 2294
    Illegal XbaI site found at 1459
    Illegal PstI site found at 874
    Illegal PstI site found at 1421
    Illegal PstI site found at 2384
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1416
    Illegal EcoRI site found at 2294
    Illegal XbaI site found at 1459
    Illegal PstI site found at 874
    Illegal PstI site found at 1421
    Illegal PstI site found at 2384
    Illegal NgoMIV site found at 3149
  • 1000
    COMPATIBLE WITH RFC[1000]