Difference between revisions of "Part:BBa K590052"

Latest revision as of 22:36, 28 September 2011

Aldehyde Decarbonylase to Acyl-ACP Reductase (ADC-AAR) Fusion

This part encodes a direct gene fusion product of the two enzymes, Aldehyde Decarbonylase (ADC) and Acyl-ACP Reductase (AAR), in a linear configuration with a glycine-serine linker for co-localization.

Usage and Biology

We expected this would optimize alkane production developed by the 2011 University of Washington iGEM Team, by bringing ADC and AAR closer together. Contrary to what was expected, our gas chromatography-mass spectroscopy (GC-MS) data demonstrated that direct fusion has a negative effect on alkane production relative to concurrent work with independent expression constructs. The functional assembly was put under a high-copy constitutive promoter and can be found under the BBa_K590049 part page.

For more information, refer to [http://2011.igem.org/Team:Washington 2011 University of Washington iGEM Team wiki].

Direct fusion of two proteins using a glycine-serine linker.

GC-MS data shows that this configuration did not increase alkane production. For more info, refer to 2011 UW iGEM Team wiki

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K590052 short</partinfo>
-Synechococcus elongatus PCC7942 Aldehyde Decarbonylase [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590031 ADC] to Acyl-ACP Reductase [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590032 AAR] (ADC-AAR) direct gene fusion w/ linker for co-localization optimization of [[Part:BBa_K590031]] alkane production developed by the [http://2011.igem.org/Team:Washington 2011 University of Washington iGEM Team].
+This part encodes a direct gene fusion product of the two enzymes, [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590031 Aldehyde Decarbonylase] (ADC) and [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590032 Acyl-ACP Reductase] (AAR), in a linear configuration with a glycine-serine linker for co-localization.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+We expected this would optimize alkane production developed by the 2011 University of Washington iGEM Team, by bringing ADC and AAR closer together. Contrary to what was expected, our gas chromatography-mass spectroscopy (GC-MS) data demonstrated that direct fusion has a negative effect on alkane production relative to concurrent work with independent expression constructs. The functional assembly was put under a high-copy constitutive promoter and can be found under the <partinfo>BBa_K590049</partinfo> part page.
+For more information, refer to [http://2011.igem.org/Team:Washington 2011 University of Washington iGEM Team wiki].
+[[Image:Washington2011_Direct_Fusion_Localization_Image.png|left|430px|thumb|Direct fusion of two proteins using a glycine-serine linker.]]
+[[Image:Washington2011_Alkane_Production_Localization_Graph1.png‎ |right|430px|thumb|GC-MS data shows that this configuration did not increase alkane production. For more info, refer to 2011 UW iGEM Team wiki]]
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