Difference between revisions of "Part:BBa K620001:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | Had to | + | Had to Quikchange to remove PstI sites within the gene. |
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===Source=== | ===Source=== | ||
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===References=== | ===References=== | ||
+ | *Lewis, J., Mantovani, Simone M., Fu, Y. (2010). Combinatorial Alanine Substitution Enables Rapid Optimization ofCytochrome P450BM3for Selective Hydroxylation of Large Substrates. ChemBioChem Volume 11, Issue 18, 2502–2505. [http://onlinelibrary.wiley.com/doi/10.1002/cbic.201000565/abstract http://onlinelibrary.wiley.com/doi/10.1002/cbic.201000565/abstract] |
Latest revision as of 23:54, 28 September 2011
WT-F87A (p450)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 75
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1219
Illegal SapI.rc site found at 2242
Illegal SapI.rc site found at 2842
Design Notes
Had to Quikchange to remove PstI sites within the gene.
Source
This part came from Dr. Frances Arnold at Caltech.
References
- Lewis, J., Mantovani, Simone M., Fu, Y. (2010). Combinatorial Alanine Substitution Enables Rapid Optimization ofCytochrome P450BM3for Selective Hydroxylation of Large Substrates. ChemBioChem Volume 11, Issue 18, 2502–2505. [http://onlinelibrary.wiley.com/doi/10.1002/cbic.201000565/abstract http://onlinelibrary.wiley.com/doi/10.1002/cbic.201000565/abstract]