Difference between revisions of "Part:BBa K592004:Design"
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Ytva comes from the genome of ''Bacillus subtilis''. | Ytva comes from the genome of ''Bacillus subtilis''. | ||
| − | FixL derives from the genome of ''Bradyrhizobium japonicum''. | + | FixL derives from the genome of ''Bradyrhizobium japonicum''. |
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| + | The DNA was synthesized by GenScript and been through quality control. Therefore, this construct was never sequenced on its own. However, it has been sequenced after assembling with other components, notably in <partinfo>BBa_S04616</partinfo>. | ||
===References=== | ===References=== | ||
Latest revision as of 22:54, 21 September 2011
YF1 blue-light sensor protein
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 544
Illegal NgoMIV site found at 616
Illegal NgoMIV site found at 706
Illegal NgoMIV site found at 724
Illegal AgeI site found at 258 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 157
Design Notes
The sequence information was provided in the article by Moglich and Moffat referenced below. The physical DNA was synthesized by GenScript. The sequence uses the wild-type codon usage, no codon optimization has been done.
Source
Ytva comes from the genome of Bacillus subtilis.
FixL derives from the genome of Bradyrhizobium japonicum.
The DNA was synthesized by GenScript and been through quality control. Therefore, this construct was never sequenced on its own. However, it has been sequenced after assembling with other components, notably in BBa_S04616.
References
Moglich A, Ayers RA and Moffat K. (2009) Design and Signaling Mechanism of Light-Regulated Histidine Kinases. J. Mol. Bio. 385, 5, 1433-1444.