Difference between revisions of "Part:BBa K678049"
 
(8 intermediate revisions by the same user not shown)
Line 2: Line 2:
 
<partinfo>BBa_K678049 short</partinfo>
 
<partinfo>BBa_K678049 short</partinfo>
  
−
JEJAM1 is a plasmid intended for transient transfection of mammalian cells. JEJAM1 consist of the strong constitutive promoter, ''CMV'', the green fluorescence protein (GFP), the ''BGK'' polyA terminator and the ''hygR'' marker cassette used for selection, an ori for propagation in ''E.coli'' and and ampR resistance cassette (Figure below).
+
pJEJAM1 is a plasmid intended for transient transfection of mammalian cells. pJEJAM1 consists of the strong constitutive cytomegalovirus (CMV) promoter, green fluorescence protein (GFP), the BGH polyA, the ''hygR'' marker cassette, an ori for propagation in ''E.coli'', and an ampR resistance cassette (Figure 1).
  
 +
<br>
 +
<br>
 +
[[Image:Devices_13.png|none|500px|thumb|<b>DTU-Denmark-2 2011</b> Figure 1: Plasmid map of pJEJAM1, the figure is not drawn to scale.]]
 +
<br>
 +
<br>
 +
Expression of pJEJAM1 in the mammalian cell line U-2 OS results in a homogenous distribution of GFP in the cells (Figure 2 and Figure 3).
 +
 +
[[Image:IGEM_001.png|left|425px|thumb|<b>DTU-Denmark-2 2011</b> Figure 2: U-2 OS cells transiently transfected with pJEJAM1 expressing GFP. The white bar has a length of 20μm.]]
 +
[[Image:IGEM3.png|right|425px|thumb|<b>DTU-Denmark-2 2011</b> Figure 3: U-2 OS cells transiently transfected with pJEJAM1 expressing GFP. The white bar has a length of 20μm.]]
 +
<br clear=all>
  
  

Latest revision as of 15:45, 27 September 2011

pJEJAM1

pJEJAM1 is a plasmid intended for transient transfection of mammalian cells. pJEJAM1 consists of the strong constitutive cytomegalovirus (CMV) promoter, green fluorescence protein (GFP), the BGH polyA, the hygR marker cassette, an ori for propagation in E.coli, and an ampR resistance cassette (Figure 1).



DTU-Denmark-2 2011 Figure 1: Plasmid map of pJEJAM1, the figure is not drawn to scale.



Expression of pJEJAM1 in the mammalian cell line U-2 OS results in a homogenous distribution of GFP in the cells (Figure 2 and Figure 3).

DTU-Denmark-2 2011 Figure 2: U-2 OS cells transiently transfected with pJEJAM1 expressing GFP. The white bar has a length of 20μm.
DTU-Denmark-2 2011 Figure 3: U-2 OS cells transiently transfected with pJEJAM1 expressing GFP. The white bar has a length of 20μm.



Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2216
    Illegal PstI site found at 2306
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2216
    Illegal PstI site found at 2306
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2216
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2216
    Illegal PstI site found at 2306
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2216
    Illegal PstI site found at 2306
    Illegal NgoMIV site found at 3071
  • 1000
    COMPATIBLE WITH RFC[1000]