Difference between revisions of "Part:BBa K649300"

Latest revision as of 14:12, 28 October 2011

Arginase (rocF)

This part has only rocF obtained from B.subtilis 168 by colony-PCR.
rocF is the cording region of Arginase(EC:3.5.3.1), catalyzing the reaction from arginine and water to urea and ornithine.

Original rocF has two EcoRI sites in ORF, so we changed these sites.

To characterize this part, we constructed the composite part(BBa_K649301). To know detailed infomation, please click here.

Urea concentration in growth media 1 hour after IPTG induction.
This work is done by Natsuki Kubo.

Introduction of BBa_K649301 containing arginase coding gene (rocF) led to production of some urea in strain MG1655 (argR +).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 137
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K649300 short</partinfo>
-This part has only rocF obtained from B.subtilis 168 by colony-PCR.
+This part has only rocF obtained from <span style="font-style:italic;">B.subtilis</span> 168 by colony-PCR.<br />
-rocF is the cording region of Arginase, catalyzing the reaction from arginine to urea and ornithine.
+rocF is the cording region of Arginase(EC:3.5.3.1), catalyzing the reaction from arginine and water to urea and ornithine.<br />
 Original rocF has two EcoRI sites in ORF, so we changed these sites.
+To characterize this part, we constructed the composite part(BBa_K649301). To know detailed infomation, please click [https://parts.igem.org/Part:BBa_K649301 here].
+[[Image:RocF_on_3K3_MG_only.png|thumb|center|400px|Urea concentration in growth media 1 hour after IPTG induction.<br />
+This work is done by Natsuki Kubo.]]
+Introduction of BBa_K649301 containing arginase coding gene (rocF) led to production of some urea in strain MG1655 (argR +).
 <!-- Add more about the biology of this part here