Difference between revisions of "Part:BBa K590007"

Latest revision as of 06:10, 22 September 2011

mamQRB, from Magnetospirillum magneticum

This part includes gene mamQ, mamR, and mamB, which were extracted from the genome of Magnetospirillum magneticum strain AMB-1 by [http://2011.igem.org/Team:Washington UW iGEM Team 2011].

Usage and Biology

They are gene AMB0972, AMB0973, and AMB0974 respectively. mamQ was reported to be important for formation/maintenance of magnetosome membranes; mam R plays a role in controlling both particle number and size of magnetite cyrstals; mamB has indirect role in magnetosome membrane invagination and biomineralization; magnetosome compartment formation.

Below is the gel image of mamQRB (2029bp):

1kb Ladder (left), mamQRB (right)

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 1813
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 1813
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 990
Illegal BglII site found at 1005
Illegal BglII site found at 1574
Illegal XhoI site found at 1067
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 1813
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 1813
Illegal NgoMIV site found at 836
Illegal NgoMIV site found at 1365
Illegal NgoMIV site found at 1973
Illegal AgeI site found at 1631
1000
COMPATIBLE WITH RFC[1000]

@@ Line 7: / Line 7: @@
 They are gene AMB0972, AMB0973, and AMB0974 respectively. mamQ was reported to be important for formation/maintenance of magnetosome membranes; mam R plays a role in controlling both particle number and size of magnetite cyrstals; mamB has indirect role in magnetosome membrane invagination and biomineralization; magnetosome compartment formation.
+Below is the gel image of mamQRB (2029bp):
+[[Image:Igem2011 QRB gel.png|center|thumb|1kb Ladder (left), mamQRB (right)]]
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