Difference between revisions of "Part:BBa K649202:Design"
| (3 intermediate revisions by the same user not shown) | |||
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K649202 short</partinfo> | <partinfo>BBa_K649202 short</partinfo> | ||
| Line 7: | Line 6: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | + | PlacIQ + lox2272 + RBS + mRFP + T7 terminator + lox2272 + RBS + GFP + T7 | |
| − | + | ||
| − | + | ||
===Source=== | ===Source=== | ||
| − | + | The forward lox2272 is synthetic oligo and the following lox2272 was produced by PCR (template BBa_J04450). The information of lox2272 sequence was gain from the reference below. | |
===References=== | ===References=== | ||
| + | Site-directed integration of the cre gene mediated by Cre recombinase using a combination of mutant lox sites, Kimi Araki ''et al'', Nucleic Acids Research 2002. | ||
Latest revision as of 15:28, 4 October 2011
PlacIQ-lox71-rfp-lox66-gfp
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1016
Illegal BamHI site found at 14 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 686
Illegal AgeI site found at 798 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1700
Design Notes
PlacIQ + lox2272 + RBS + mRFP + T7 terminator + lox2272 + RBS + GFP + T7
Source
The forward lox2272 is synthetic oligo and the following lox2272 was produced by PCR (template BBa_J04450). The information of lox2272 sequence was gain from the reference below.
References
Site-directed integration of the cre gene mediated by Cre recombinase using a combination of mutant lox sites, Kimi Araki et al, Nucleic Acids Research 2002.