Difference between revisions of "Part:BBa K611013"

Latest revision as of 01:26, 25 September 2011

pLacI Characterization Construct

This part is used to characterize mutants of BBa_R0010 (pLacI). A mutant of BBa_R0010 can be ligated before this part (on the 5' end) by cutting this plasmid at EcoRI and XbaI and the mutant promoter at EcoRI and SpeI. Several of the mutant's properties can then be measured through the expression of GFP. Certain characteristics of the mutant, such as the binding of the LacI repressor, can be measured after inducing the pBad promoter with arabinose. Concentration of the repressor can be varied through different arabinose concentrations as long as AraC is present.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1008
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 2186
Illegal BamHI site found at 948
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 662

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 __NOTOC__
 <partinfo>BBa_K611013 short</partinfo>
-This part is used to characterize mutants of BBa_R0010 (pLacI). By adding a mutant of BBa_R0010 before this part, one can measure several of its properties through the expression of GFP. Certain characteristics of the mutant, such as the binding of the LacI repressor, can be measured after inducing the pBad promoter with arabinose. Concentration of the repressor can be varied through different arabinose concentrations as long as AraC is present.
+This part is used to characterize mutants of BBa_R0010 (pLacI). A mutant of BBa_R0010 can be ligated before this part (on the 5' end) by cutting this plasmid at EcoRI and XbaI and the mutant promoter at EcoRI and SpeI. Several of the mutant's properties can then be measured through the expression of GFP. Certain characteristics of the mutant, such as the binding of the LacI repressor, can be measured after inducing the pBad promoter with arabinose. Concentration of the repressor can be varied through different arabinose concentrations as long as AraC is present.
 <!-- Add more about the biology of this part here