Difference between revisions of "Part:BBa K626005:Design"

 
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===Design Notes===
 
===Design Notes===
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The reconstruction of the DBD, LBD and Backbone will be done with RFC 25.
  
 
===Source===
 
===Source===
  
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Arteficially synthetized Ligand Binding domain of C. elegans nuclear receptor NHR-64. This part is placed into pSB1C3 carrier vector after the Gal4 artificial DNA binding domain.
  
 
===References===
 
===References===
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Liang B, Ferguson K, Kadyk L, Watts JL, 2010. The Role of Nuclear Receptor NHR-64 in Fat Storage Regulation in Caenorhabditis elegans. PLoS ONE 5(3): e9869. doi:10.1371/journal.pone.0009869

Latest revision as of 20:47, 19 September 2011

Gal4 - NHR64


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 218
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 137
    Illegal BsaI.rc site found at 627


Design Notes

The reconstruction of the DBD, LBD and Backbone will be done with RFC 25.

Source

Arteficially synthetized Ligand Binding domain of C. elegans nuclear receptor NHR-64. This part is placed into pSB1C3 carrier vector after the Gal4 artificial DNA binding domain.

References

Liang B, Ferguson K, Kadyk L, Watts JL, 2010. The Role of Nuclear Receptor NHR-64 in Fat Storage Regulation in Caenorhabditis elegans. PLoS ONE 5(3): e9869. doi:10.1371/journal.pone.0009869