Difference between revisions of "Part:BBa K537015"
 
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This composite BioBrick begins with a strong, constitutively active promoter of E.coli (BBa_J23119) followed by an atrazine riboswitch riboswitch (Sinha et al, 2010) which is fused to CheZ and monomeric Red fluorescent reporter protein without stop codons in between. The Freiberg iGEM 2007 BioBrick 3.0 fusion protein assembly was used to construct this BioBrick. The atrazine riboswitch-CheZ fusion was constructed via PCR. This part ends with a standard double terminator transcriptional terminator for E.coli (BBa_B0015).
 
This composite BioBrick begins with a strong, constitutively active promoter of E.coli (BBa_J23119) followed by an atrazine riboswitch riboswitch (Sinha et al, 2010) which is fused to CheZ and monomeric Red fluorescent reporter protein without stop codons in between. The Freiberg iGEM 2007 BioBrick 3.0 fusion protein assembly was used to construct this BioBrick. The atrazine riboswitch-CheZ fusion was constructed via PCR. This part ends with a standard double terminator transcriptional terminator for E.coli (BBa_B0015).
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Note: All scar sites have been excluded in the annotations. There is a scar site between the Promoter:CheZ-mRFP fusion and CheZ-mRFP fusion:Double terminator. The scar sequence is as follows: ACTAGA
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A Freiburg fusion scar site exists between the CheZ and mRFP sequence. The sequence is as follows: ACCGGC
  
 
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Latest revision as of 18:15, 19 September 2011

Promoter-Atrazine riboswitch-CheZ-mCherry-Double terminator

This composite BioBrick begins with a strong, constitutively active promoter of E.coli (BBa_J23119) followed by an atrazine riboswitch riboswitch (Sinha et al, 2010) which is fused to CheZ and monomeric Red fluorescent reporter protein without stop codons in between. The Freiberg iGEM 2007 BioBrick 3.0 fusion protein assembly was used to construct this BioBrick. The atrazine riboswitch-CheZ fusion was constructed via PCR. This part ends with a standard double terminator transcriptional terminator for E.coli (BBa_B0015).

Note: All scar sites have been excluded in the annotations. There is a scar site between the Promoter:CheZ-mRFP fusion and CheZ-mRFP fusion:Double terminator. The scar sequence is as follows: ACTAGA

A Freiburg fusion scar site exists between the CheZ and mRFP sequence. The sequence is as follows: ACCGGC

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 44
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1314
    Illegal AgeI site found at 1426
  • 1000
    COMPATIBLE WITH RFC[1000]