Difference between revisions of "Part:BBa K584004"
 
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<partinfo>BBa_K584004 short</partinfo>
 
<partinfo>BBa_K584004 short</partinfo>
  
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This part was designed to test the functionality of the promotor region. We cloned the HybB promoter via PCR using the [https://parts.igem.org/Part:BBa_K410000 K410000] as a template using the primers below -
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<u><b>Characterization</b></u>
  
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hyb-FW: CCGGAATTCGCGGCCGCTTCTAGAGCGCCGCTATGGACTGGATAAAG 
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<p align="justify">The <b>[http://2011.igem.org/Team:KULeuven K.U.Leuven 2011 iGEM Team]</b> has <b><u>extensively characterized</u></b> the <b><u>existing Biobrick part</u></b> [https://parts.igem.org/wiki/index.php?title=Part:BBa_J45503 hybB Cold Shock Promoter(BBa_J45503)].</p>
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hyb-RV: AAAACTGCAGCGGCCGCTACTAGTATGCTACTTAACCCCATGGTGG   
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The detailed qualitative and quantitative <u><b>[https://parts.igem.org/Part:BBa_J45503:Experience#User_Reviews review]</b></u> has been added into the experience page of the BBa_J45503 hybB Cold Shock Promoter.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 10:29, 21 September 2011

HybB promoter + GFP generator

Characterization

The [http://2011.igem.org/Team:KULeuven K.U.Leuven 2011 iGEM Team] has extensively characterized the existing Biobrick part hybB Cold Shock Promoter(BBa_J45503).

The detailed qualitative and quantitative review has been added into the experience page of the BBa_J45503 hybB Cold Shock Promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1064