Difference between revisions of "Part:BBa K592015:Design"
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We found this design very useful when assembling components that contain RFP, mCherry, etc. The assembled parts turn red and are not recognizable from the background insert(BBa_J04450) if all colonies becomes red after transformation. Luckily, very few genes produce things that turn blue/purple, which makes our construct extremely useful. | We found this design very useful when assembling components that contain RFP, mCherry, etc. The assembled parts turn red and are not recognizable from the background insert(BBa_J04450) if all colonies becomes red after transformation. Luckily, very few genes produce things that turn blue/purple, which makes our construct extremely useful. | ||
| − | B0014 instead of B0015 in order to avoid unwanted primer binding, etc. J23101 promoter to ensure strong and constitutive expression at all times. | + | B0014 instead of B0015 in order to avoid unwanted primer binding, etc. J23101 promoter to ensure strong and constitutive expression at all times. |
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===Source=== | ===Source=== | ||
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===References=== | ===References=== | ||
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| + | =Improvement by JNFLS2020= | ||
| + | ==Overview:== | ||
| + | <br/> | ||
Latest revision as of 16:22, 20 October 2021
amilCP Blue Coding Device
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We found this design very useful when assembling components that contain RFP, mCherry, etc. The assembled parts turn red and are not recognizable from the background insert(BBa_J04450) if all colonies becomes red after transformation. Luckily, very few genes produce things that turn blue/purple, which makes our construct extremely useful.
B0014 instead of B0015 in order to avoid unwanted primer binding, etc. J23101 promoter to ensure strong and constitutive expression at all times.
Source
amilCP comes from the genome of Acropora millepora. All other parts are assembled from the iGEM 2011 distribution kit.
References
Improvement by JNFLS2020
Overview: