Difference between revisions of "Part:BBa K584021"
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<partinfo>BBa_K584021 short</partinfo> | <partinfo>BBa_K584021 short</partinfo> | ||
− | This part contains the Ice Nucleating Protein coding sequence. The ice nucleation protein (INP) is a glycosyl phosphatidylinositol anchored outer membrane protein found in certain Gram-negative bacteria. '''inaZ''' is the gene responsible for producing the ice nucleation protein. | + | <p align="justify">This part contains the Ice Nucleating Protein coding sequence. The ice nucleation protein (INP) is a glycosyl phosphatidylinositol anchored outer membrane protein found in certain Gram-negative bacteria. '''inaZ''' is the gene responsible for producing the ice nucleation protein.</p> |
− | We would like to thank [http://www.academicbiography.uwo.ca/profile.php?&n=ggloor Prof. Dr. Gregory Gloor ], for providing us with a plasmid (pUC1813ICE) which contains the ice nucleating protein gene '''inaZ'''. | + | <p align="justify">We would like to thank [http://www.academicbiography.uwo.ca/profile.php?&n=ggloor Prof. Dr. Gregory Gloor ], for providing us with a plasmid (pUC1813ICE) which contains the ice nucleating protein gene '''inaZ'''.</p> |
− | To know more about the inaZ, it is suggested to read this [http://www.ncbi.nlm.nih.gov/pubmed/10933828 article] describing the usage of pUC1813ICE with the '''inaZ'''. | + | <p align="justify">To know more about the inaZ, it is suggested to read this [http://www.ncbi.nlm.nih.gov/pubmed/10933828 article] describing the usage of pUC1813ICE with the '''inaZ'''.</p> |
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+ | The UGent Belgium 2016 iGEM team improved this part by removing 2 BsaI restriction sites in order to make it compatible with Golden Gate assembly/MoClo, creating [[Part:BBa_K1896011|BBa_K1896011]]. | ||
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Latest revision as of 15:08, 19 October 2016
Ice Nucleating Protein Gene - inaZ
This part contains the Ice Nucleating Protein coding sequence. The ice nucleation protein (INP) is a glycosyl phosphatidylinositol anchored outer membrane protein found in certain Gram-negative bacteria. inaZ is the gene responsible for producing the ice nucleation protein.
We would like to thank [http://www.academicbiography.uwo.ca/profile.php?&n=ggloor Prof. Dr. Gregory Gloor ], for providing us with a plasmid (pUC1813ICE) which contains the ice nucleating protein gene inaZ.
To know more about the inaZ, it is suggested to read this [http://www.ncbi.nlm.nih.gov/pubmed/10933828 article] describing the usage of pUC1813ICE with the inaZ.
The UGent Belgium 2016 iGEM team improved this part by removing 2 BsaI restriction sites in order to make it compatible with Golden Gate assembly/MoClo, creating BBa_K1896011.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 646
Illegal NgoMIV site found at 2158
Illegal NgoMIV site found at 2302
Illegal NgoMIV site found at 2566
Illegal NgoMIV site found at 2710
Illegal NgoMIV site found at 2758
Illegal NgoMIV site found at 2878
Illegal AgeI site found at 417
Illegal AgeI site found at 1822 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 592
Illegal BsaI.rc site found at 3338
Illegal SapI.rc site found at 238