Difference between revisions of "Part:BBa K528000"
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*Michał Lower | *Michał Lower | ||
*Ania Olchowik | *Ania Olchowik | ||
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===Measurements=== | ===Measurements=== | ||
| − | Using this construct we have determined that | + | Using this construct we have determined that strength of BBa_B0031 with mOrrange is 69,73% relative to BBa_B0034 with mOrrange. |
| − | All our measurement results are summarized on the chart below: | + | All our mORANGE measurement results are summarized on the chart below: |
https://static.igem.org/mediawiki/parts/7/79/Wyk5.png | https://static.igem.org/mediawiki/parts/7/79/Wyk5.png | ||
Latest revision as of 21:48, 18 September 2011
RBS measurement device J23100 B0031 mORANGE
This device was used to determine BBa_B0031 strength relative to BBa_B0034.
Construct design
The device consists of BBa_K299501 expression vector J23100+B0031. E2050 mORANGE was used as reporter protein.
Authors
Clonning:
- Paweł Urbański
- Elżbieta Jankowska
- Krzysztof Szczepaniak
- Dorota Kaja Sabat
The lab work was supervised by:
- Michał Lower
- Ania Olchowik
Measurements
Using this construct we have determined that strength of BBa_B0031 with mOrrange is 69,73% relative to BBa_B0034 with mOrrange.
All our mORANGE measurement results are summarized on the chart below:
For more info please look at Team Warsaw 2011 [http://2011.igem.org/Team:Warsaw/RBSmeasurement].
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 826
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]