Difference between revisions of "Part:BBa K537008:Design"

(→‎Design Notes)
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===References===
 
===References===
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1. Sinha J., Reyes S.J., and Gallivan J.P. Reprogramming Bacteria to Seek and Destroy a Herbicide. 2010, Nat Chem Biol. 6(6): 464–470

Latest revision as of 13:39, 18 September 2011

Atrazine Riboswitch-mRFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 9
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 640
    Illegal AgeI site found at 752
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This biobrick part represents a composite of a atrazine riboswitch upstream of a mRFP1 fluorescent protein reporter (derived from part BBa_E1010).

Previous teams: Lethbridge 2007, Lethbridge 2009, and NYMU Taipei 2010 all considered strategies for making the riboswitch a separate biobrick, however standard assembly techniques are inadequate since the sequence distance between the ATG, RBS and aptamer domain of the riboswitch are critical. This is why we decided to synthesise this construct via PCR. Primers were designed such that the PCR amplification resulted in an atrazine riboswitch closely fused to the mRFP reporter protein.

Source

The mRFP1 fluorescent reporter is derived from part BBa_E1010

References

1. Sinha J., Reyes S.J., and Gallivan J.P. Reprogramming Bacteria to Seek and Destroy a Herbicide. 2010, Nat Chem Biol. 6(6): 464–470