Difference between revisions of "Part:BBa K528000"
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This device was used to determine BBa_B0031 strength relative to BBa_B0034.
 
This device was used to determine BBa_B0031 strength relative to BBa_B0034.
  
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<b><font size="4">Construct design</font></b>
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===Construct design===
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<p align=justify>
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The device consists of BBa_K299501 expression vector J23100+B0031. E2050 mORANGE was used as reporter protein.  
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The device consists of BBa_K299501 expression vector J23100+B0031. E2050 mORANGE was used as reporter protein. </p>
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===Authors===
 
===Authors===
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*Michał Lower
 
*Michał Lower
 
*Ania Olchowik
 
*Ania Olchowik
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Flow cytometer measurements done by Cherry Moreno
 
  
 
===Measurements===
 
===Measurements===
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Using this construct we have determined that {{{name}}} strength is '''{{{strength}}}''' relative to <partinfo>B0034</partinfo>
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Using this construct we have determined that strength of BBa_B0031 with mOrrange is 69,73% relative to BBa_B0034 with mOrrange.
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All our mORANGE measurement results are summarized on the chart below:
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https://static.igem.org/mediawiki/parts/7/79/Wyk5.png
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For more info please look at Team Warsaw 2011 [http://2011.igem.org/Team:Warsaw/RBSmeasurement].
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 21:48, 18 September 2011

RBS measurement device J23100 B0031 mORANGE

This device was used to determine BBa_B0031 strength relative to BBa_B0034.

Construct design

The device consists of BBa_K299501 expression vector J23100+B0031. E2050 mORANGE was used as reporter protein.

Authors

Clonning:

  • Paweł Urbański
  • Elżbieta Jankowska
  • Krzysztof Szczepaniak
  • Dorota Kaja Sabat

The lab work was supervised by:

  • Michał Lower
  • Ania Olchowik

Measurements

Using this construct we have determined that strength of BBa_B0031 with mOrrange is 69,73% relative to BBa_B0034 with mOrrange.

All our mORANGE measurement results are summarized on the chart below:

Wyk5.png

For more info please look at Team Warsaw 2011 [http://2011.igem.org/Team:Warsaw/RBSmeasurement].


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 826
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]