Difference between revisions of "Part:BBa K528000"

Latest revision as of 21:48, 18 September 2011

RBS measurement device J23100 B0031 mORANGE

This device was used to determine BBa_B0031 strength relative to BBa_B0034.

The device consists of BBa_K299501 expression vector J23100+B0031. E2050 mORANGE was used as reporter protein.

Clonning:

The lab work was supervised by:

Using this construct we have determined that strength of BBa_B0031 with mOrrange is 69,73% relative to BBa_B0034 with mOrrange.

All our mORANGE measurement results are summarized on the chart below:

For more info please look at Team Warsaw 2011 [http://2011.igem.org/Team:Warsaw/RBSmeasurement].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 826
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 This device was used to determine BBa_B0031 strength relative to BBa_B0034.
-<b><font size="4">Construct design</font></b>
+===Construct design===
-<p align=justify>
+The device consists of BBa_K299501 expression vector J23100+B0031. E2050 mORANGE was used as reporter protein.
-The device consists of BBa_K299501 expression vector J23100+B0031. E2050 mORANGE was used as reporter protein. </p>
 ===Authors===
@@ Line 18: / Line 17: @@
 *Michał Lower
 *Ania Olchowik
-Flow cytometer measurements done by Cherry Moreno
+===Measurements===
+Using this construct we have determined that strength of BBa_B0031 with mOrrange is 69,73% relative to BBa_B0034 with mOrrange.
+All our mORANGE measurement results are summarized on the chart below:
+https://static.igem.org/mediawiki/parts/7/79/Wyk5.png
+For more info please look at Team Warsaw 2011 [http://2011.igem.org/Team:Warsaw/RBSmeasurement].
 <!-- Add more about the biology of this part here