Difference between revisions of "Part:BBa K528000"
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<partinfo>BBa_K528000 short</partinfo>
 
<partinfo>BBa_K528000 short</partinfo>
  
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The device consists of BBa_K299501 expression vector J23100+B0031 and mORANGE E2050 as a protein.
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This device was used to determine BBa_B0031 strength relative to BBa_B0034.
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===Construct design===
 +
The device consists of BBa_K299501 expression vector J23100+B0031. E2050 mORANGE was used as reporter protein.
 +
 
 +
===Authors===
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Clonning:
 +
*Paweł Urbański
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*Elżbieta Jankowska
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*Krzysztof Szczepaniak
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*Dorota Kaja Sabat
 +
 +
The lab work was supervised by:
 +
*Michał Lower
 +
*Ania Olchowik
 +
 
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===Measurements===
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Using this construct we have determined that strength of BBa_B0031 with mOrrange is 69,73% relative to BBa_B0034 with mOrrange.
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All our mORANGE measurement results are summarized on the chart below:
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https://static.igem.org/mediawiki/parts/7/79/Wyk5.png
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For more info please look at Team Warsaw 2011 [http://2011.igem.org/Team:Warsaw/RBSmeasurement].
  
  
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<b>General info</b><p align=justify> In order to fine-tune expression of genes used in our project we have conducted measurement of various ribosome binding sites included in 2011 spring distribution. Our list of measured parts includes YFP E0030 (part 723 bp  pSB1AK3), GFP E0020 (part 723bp  pSB1A2), RFP E1010 (part 681 bp  pSB2K3), mOrange E2050, MATSG—GSGTAlinkers (part 744bp  pSB2K3), GFP    E0040  GFPmut3b (720 bp pSB1A2). Also DNA of SUPERFOLDER GFP, which was included in this distribution as a bigger part with RBS and promotor. We obtained coding sequence of SUPERFOLDER GFP using PCR method.</p>
 
  
 
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Latest revision as of 21:48, 18 September 2011

RBS measurement device J23100 B0031 mORANGE

This device was used to determine BBa_B0031 strength relative to BBa_B0034.

Construct design

The device consists of BBa_K299501 expression vector J23100+B0031. E2050 mORANGE was used as reporter protein.

Authors

Clonning:

  • Paweł Urbański
  • Elżbieta Jankowska
  • Krzysztof Szczepaniak
  • Dorota Kaja Sabat

The lab work was supervised by:

  • Michał Lower
  • Ania Olchowik

Measurements

Using this construct we have determined that strength of BBa_B0031 with mOrrange is 69,73% relative to BBa_B0034 with mOrrange.

All our mORANGE measurement results are summarized on the chart below:

Wyk5.png

For more info please look at Team Warsaw 2011 [http://2011.igem.org/Team:Warsaw/RBSmeasurement].


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 826
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]