Difference between revisions of "Part:BBa K553000:Design"

(→‎Design Notes)
(→‎References)
 
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===Design Notes===
 
===Design Notes===
  
−
the part was generated by per with these primers:
+
the part was generated by using the following primers:
  
−
TraR rev 5' GTTTCTTCCTGCAGCGGCCGCTACTAGTATCAGATGAGTTTCCGCCGGATG  3'
 
−
TraR fw 5' GTTTCTTCGAATTCGCGGCCGCTTCTAGATCGACGACTGGCTGGACAAGC    3'
 
  
−
with the present protocol:
+
'''TraR fw '''
 +
 
 +
5' GTTTCTTCGAATTCGCGGCCGCTTCTAGATCGACGACTGGCTGGACAAGC    3'
 +
 
 +
'''TraR rev '''
 +
 
 +
5' GTTTCTTCCTGCAGCGGCCGCTACTAGTATCAGATGAGTTTCCGCCGGATG  3'
 +
 
 +
 
 +
pcr protocol:
  
 
95° 5' | 10x (93° 30" | 56° 30" | 72° 40") | 23x ( (93° 30" | 65° 30" | 72° 40") | 72° 7' | 4° ∞
 
95° 5' | 10x (93° 30" | 56° 30" | 72° 40") | 23x ( (93° 30" | 65° 30" | 72° 40") | 72° 7' | 4° ∞
Line 21: Line 28:
 
===Source===
 
===Source===
  
−
The gene was extracted by pcr from Agrobacterium Tumefaciens gDNA.
+
The gene was extracted by pcr from ''Agrobacterium tumefaciens'' gDNA.
  
−
http://www.ncbi.nlm.nih.gov/nucleotide/81176483?report=genbank&log$=nuclalign&blast_rank=1&RID=7BS4G4AC014.
+
http://www.ncbi.nlm.nih.gov/gene/1224220
  
 
The sequencing revealed mutations in our strain that do not affect the part functionality.
 
The sequencing revealed mutations in our strain that do not affect the part functionality.
  
 
===References===
 
===References===
 +
 +
Piper KR, Beck von Bodman S, Far- rand SK. 1993. ''Conjugation factor of Agrobacterium tumefaciens regulates Ti plasmid transfer by autoinduction''. Nature 362:448–50
 +
 +
Zhang L, Murphy PJ, Kerr A, Tate ME. 1993. ''Agrobacterium conjugation and gene regulation by N-acyl-L-homoserine lactones''. Nature 362:446–48

Latest revision as of 21:47, 20 September 2011

TraR trans-activator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

the part was generated by using the following primers:


TraR fw

5' GTTTCTTCGAATTCGCGGCCGCTTCTAGATCGACGACTGGCTGGACAAGC 3'

TraR rev

5' GTTTCTTCCTGCAGCGGCCGCTACTAGTATCAGATGAGTTTCCGCCGGATG 3'


pcr protocol:

95° 5' | 10x (93° 30" | 56° 30" | 72° 40") | 23x ( (93° 30" | 65° 30" | 72° 40") | 72° 7' | 4° ∞


The part contains also the BBa_B0034 RBS and BBa_B0015 double terminator.

Source

The gene was extracted by pcr from Agrobacterium tumefaciens gDNA.

http://www.ncbi.nlm.nih.gov/gene/1224220

The sequencing revealed mutations in our strain that do not affect the part functionality.

References

Piper KR, Beck von Bodman S, Far- rand SK. 1993. Conjugation factor of Agrobacterium tumefaciens regulates Ti plasmid transfer by autoinduction. Nature 362:448–50

Zhang L, Murphy PJ, Kerr A, Tate ME. 1993. Agrobacterium conjugation and gene regulation by N-acyl-L-homoserine lactones. Nature 362:446–48