Difference between revisions of "Part:BBa K527001"
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<partinfo>BBa_K527001 short</partinfo> | <partinfo>BBa_K527001 short</partinfo> | ||
− | This BioBrick BBa_K527001 encode the plant enzyme Cytochrome P450 CYP79A2. Cytochromes | + | This BioBrick BBa_K527001 encode the plant enzyme Cytochrome P450 CYP79A2. Cytochromes P450 are defined as heme-thiolate proteins featuring a particular spectral signature at 450 nm, thus the name. Despite the label as cytochromes, these proteins are not involved in electron transfers, but act as monooxygenases in a wide range of reactions such as epoxidation, N-dealkylation, O-dealkylation, S-oxidation and hydroxylation. |
− | CYP79A2 is an N-hydroxylase converting L-phenylalanine into phenylacetaldoxime, the precursor of | + | CYP79A2 is an N-hydroxylase converting L-phenylalanine into phenylacetaldoxime, the precursor of a cyanogenic glycoside. Oximes as the phenylacetaldoxime is generally toxic to fungi and is frequently used as a chemical fungicide. They function by inhibiting mitochondrial oxidases and thereby promote lipid peroxidation and the production of toxic reactive oxygen species. |
− | BBa_K527001 codes for the same enzyme as BBa_K527000, but is submitted in an other plasmid due to the different assembly method (Plug 'n' Play) hence the missing prefix and suffix. | + | BBa_K527001 codes for the same enzyme as BBa_K527000, but is submitted in an other plasmid (BBa_K678023) due to the different assembly method (Plug 'n' Play) hence the missing prefix and suffix. |
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<partinfo>BBa_K527001 parameters</partinfo> | <partinfo>BBa_K527001 parameters</partinfo> | ||
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+ | ===References=== | ||
+ | * Wittstock U and Halkier B A (2000). ''Cytochrome P450 CYP79A2 from Arabidopsis thaliana L. Catalyzes the Conversion of L-Phenylalanine to Phenylacetaldoxime in the Biosynthesis of Benzylglucosinolate''. The Journal of Biological Chemistry Vol. 275, No. 19, Issue of May 12, pp. 14659–14666. | ||
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+ | * Møller B L (2010). ''Dynamic Metabolons.'' Science Vol. 330, 1328-1329. |
Latest revision as of 14:18, 18 September 2011
Biosynthesis/Oximes/CYP79A2/Plug 'n' Play
This BioBrick BBa_K527001 encode the plant enzyme Cytochrome P450 CYP79A2. Cytochromes P450 are defined as heme-thiolate proteins featuring a particular spectral signature at 450 nm, thus the name. Despite the label as cytochromes, these proteins are not involved in electron transfers, but act as monooxygenases in a wide range of reactions such as epoxidation, N-dealkylation, O-dealkylation, S-oxidation and hydroxylation.
CYP79A2 is an N-hydroxylase converting L-phenylalanine into phenylacetaldoxime, the precursor of a cyanogenic glycoside. Oximes as the phenylacetaldoxime is generally toxic to fungi and is frequently used as a chemical fungicide. They function by inhibiting mitochondrial oxidases and thereby promote lipid peroxidation and the production of toxic reactive oxygen species.
BBa_K527001 codes for the same enzyme as BBa_K527000, but is submitted in an other plasmid (BBa_K678023) due to the different assembly method (Plug 'n' Play) hence the missing prefix and suffix.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 165
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 432
References
- Wittstock U and Halkier B A (2000). Cytochrome P450 CYP79A2 from Arabidopsis thaliana L. Catalyzes the Conversion of L-Phenylalanine to Phenylacetaldoxime in the Biosynthesis of Benzylglucosinolate. The Journal of Biological Chemistry Vol. 275, No. 19, Issue of May 12, pp. 14659–14666.
- Møller B L (2010). Dynamic Metabolons. Science Vol. 330, 1328-1329.