Difference between revisions of "Part:BBa K553008:Experience"

 
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===Download Datasheet===
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__NOTOC__
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<partinfo>BBa_K553008 parameters</partinfo>
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
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<html><div class="table_cell"><center><a title="BBa_K553008" target="_blank" href="https://static.igem.org/mediawiki/parts/2/23/K553008_V.pdf"><img src="https://static.igem.org/mediawiki/parts/d/da/Units-BBa_K553008.jpg"></a></center></div></html>
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how you used this part and how it worked out.
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===Applications of BBa_K553008===
 
===Applications of BBa_K553008===
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UNITS_Trieste Synbiome project
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bacteria Intra-strain communication:
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An E. Coli containing BBa_K553010 constitutively produces the OC12 AHL quorum sensing molecule (Strain A). The ammount of this molecule is enough to induce the activation of part BBa_K553008 (production of OC8 AHL) in a co-cultivated bacteria (strain B).
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The following assay demonstrates that the organic extract of a co-culture media contains a considerable ammount of OC8.
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OC8 AHL standards used: 1uM; 100nM; 10nM; 1nM
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[[Image:Coculture_assay.jpg‎]]
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The diffusion from the center of the OC8 AHL generate, in fonction of its concentration, the activation of a biosensor (A. tumefacines) in Xgal LB agar dishes.
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Here the HPLC-MS/MS analysis of the co-culture of the strain A and B.
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Even a very linle production of OC12 is able to induce a good production of OC8.
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[[Image:Ms_co-culture_.png]]
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OC8 signal @ 10 min; OC12 signial @ 12 min
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blue: standards: 1uM 5uL loaded
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black: medium background, 40x concentrated organic extract 12,5 uL loaded
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red: co-culture sample, 40x concentrated organic extract 12,5 uL loaded
  
 
===User Reviews===
 
===User Reviews===

Latest revision as of 10:35, 1 November 2011

Download Datasheet

Applications of BBa_K553008

UNITS_Trieste Synbiome project

bacteria Intra-strain communication: An E. Coli containing BBa_K553010 constitutively produces the OC12 AHL quorum sensing molecule (Strain A). The ammount of this molecule is enough to induce the activation of part BBa_K553008 (production of OC8 AHL) in a co-cultivated bacteria (strain B).

The following assay demonstrates that the organic extract of a co-culture media contains a considerable ammount of OC8.

OC8 AHL standards used: 1uM; 100nM; 10nM; 1nM

Coculture assay.jpg

The diffusion from the center of the OC8 AHL generate, in fonction of its concentration, the activation of a biosensor (A. tumefacines) in Xgal LB agar dishes.

Here the HPLC-MS/MS analysis of the co-culture of the strain A and B.

Even a very linle production of OC12 is able to induce a good production of OC8.

Ms co-culture .png

OC8 signal @ 10 min; OC12 signial @ 12 min

blue: standards: 1uM 5uL loaded black: medium background, 40x concentrated organic extract 12,5 uL loaded red: co-culture sample, 40x concentrated organic extract 12,5 uL loaded

User Reviews

UNIQb595b2ab006cd78b-partinfo-00000002-QINU UNIQb595b2ab006cd78b-partinfo-00000003-QINU