Difference between revisions of "Part:BBa K590053"
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<partinfo>BBa_K590053 short</partinfo>
 
<partinfo>BBa_K590053 short</partinfo>
  
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This part consists of [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590032 Acyl-ACP Reductase], ribosomal binding site, [https://parts.igem.org/Part:BBa_K590031 Aldehyde Decarbonylase] under the control of high consititutive promoter for alkane production.  
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This part consists of [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590032 Acyl-ACP Reductase], ribosomal binding site, [https://parts.igem.org/Part:BBa_K590031 Aldehyde Decarbonylase] under the control of high constitutive promoter for alkane production.  
  
 
===Usage and Biology===
 
===Usage and Biology===
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[[Image:Washington_Redesign_Data2.png|left|400px|thumb|Data collected from In Vivo Assay developed by University of Washington iGEM Team]]
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When wild type Aldehyde Decarbonylase is co-expressed with Acyl-ACP Reductase, odd chain length alkanes is produced from the cell's fatty acid biosynthetic pathway. Alkane production is enhanced when growing expression strains using the growth conditions developed by the 2011 University of Washington iGEM Team. For more information, refer to the [http://2011.igem.org/Team:Washington 2011 UW iGEM wiki].
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[[Image:Washinton_2011_Optimization_Quant.png|left|450px|thumb|Wild type ADC and AAR are co-expressed and GC-MS quantification of this co-expression shows greater alkane production under optimized conditions.]]
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[[Image:Washington_Redesign_Data2.png|right|430px|thumb|Mutant ADCs are co-expressed with AARs under optimized conditions.]]
  
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When AAR and ADC are co-expressed, they produce odd chain length alkanes from the cell's fatty acid biosynthetic pathway. Alkane production is enhanced when growing expression strains using the growth conditions developed by the [http://2011.igem.org/Team:Washington 2011 University of Washington iGEM Team]. 
 
  
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[[Image:Washinton_AARADCGC-MS.png|left|400px|thumb|GC plot showing that alkanes are only produced when both ADC and AAR are expressed.]]
 
  
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[[Image:Washinton_2011_Optimization_Quant.png|right|450px|thumb|GC-MS quantification shows greater alkane production under UW iGEM 2011's "optimized conditions" (see wiki for details)]]
 
  
 
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Latest revision as of 19:36, 22 September 2011

RrD_PSB1C3-High constitutive

This part consists of Acyl-ACP Reductase, ribosomal binding site, Aldehyde Decarbonylase under the control of high constitutive promoter for alkane production.

Usage and Biology

When wild type Aldehyde Decarbonylase is co-expressed with Acyl-ACP Reductase, odd chain length alkanes is produced from the cell's fatty acid biosynthetic pathway. Alkane production is enhanced when growing expression strains using the growth conditions developed by the 2011 University of Washington iGEM Team. For more information, refer to the [http://2011.igem.org/Team:Washington 2011 UW iGEM wiki].

Wild type ADC and AAR are co-expressed and GC-MS quantification of this co-expression shows greater alkane production under optimized conditions.
Mutant ADCs are co-expressed with AARs under optimized conditions.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 470
    Illegal NheI site found at 493
  • 21
    INCOMPATIBLE WITH RFC[21]
    Unknown
  • 23
    INCOMPATIBLE WITH RFC[23]
    Unknown
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 126
  • 1000
    COMPATIBLE WITH RFC[1000]