Difference between revisions of "Part:BBa K590054"
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− | [ | + | When wild type Aldehyde Decarbonylase is co-expressed with Acyl-ACP Reductase, odd chain length alkanes is produced from the cell's fatty acid biosynthetic pathway. Alkane production is enhanced when growing expression strains using the growth conditions developed by the 2011 University of Washington iGEM Team. When mutant ADC and AAR are co-expressed, alkane production profile varies. For more information, refer to the [http://2011.igem.org/Team:Washington 2011 UW iGEM wiki]. |
− | + | [[Image:Washinton_2011_Optimization_Quant.png|left|450px|thumb|Wild type ADC and AAR are co-expressed and GC-MS quantification of this co-expression shows greater alkane production under optimized conditions.]] | |
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− | [[Image: | + | |
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+ | [[Image:Washington_Redesign_Data2.png|right|430px|thumb|Mutant ADCs are co-expressed with AARs under optimized conditions.]] | ||
Latest revision as of 19:38, 22 September 2011
RrD_N25M-PSB1C3-High constitutive
This part consists of Acyl-ACP Reductase, ribosomal binding site, mutant Aldehyde Decarbonylase under the control of high consititutive promoter for alkane production.
Usage and Biology
When wild type Aldehyde Decarbonylase is co-expressed with Acyl-ACP Reductase, odd chain length alkanes is produced from the cell's fatty acid biosynthetic pathway. Alkane production is enhanced when growing expression strains using the growth conditions developed by the 2011 University of Washington iGEM Team. When mutant ADC and AAR are co-expressed, alkane production profile varies. For more information, refer to the [http://2011.igem.org/Team:Washington 2011 UW iGEM wiki].
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1584
Illegal XhoI site found at 2366 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 126
- 1000COMPATIBLE WITH RFC[1000]