Difference between revisions of "Part:BBa K515100:Experience"
 
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<img src="https://static.igem.org/mediawiki/parts/5/58/ICL_Standard_curve_salkowski.png" width=500px/>
 
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<img src="https://static.igem.org/mediawiki/parts/5/58/ICL_salkowski_cuvettes.JPG" width=400px/>
 
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<p> Figure 1 - To make a calibration curve for the Salkowski assay, LB broth with different concentrations of synthetic auxin was added to salkowski reagent and OD measured at 530 nm. The picture on the right shows the visible colour change from yellow to red with increasing auxin concentration.</p>
 
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<img src="https://static.igem.org/mediawiki/2011/b/bc/ICL_salkowskiAuxinGraph.png" width="500px"/>
 
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<p><i>Results from salkowski assay with cell filtrate (add pore size)</i></p>
 
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===Applications of BBa_K515100===
 
===Applications of BBa_K515100===
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*The enzymatic reactions of the IAM pathway produce indole-3 acetic acid (IAA) which is an important phytohormone.
 +
*Many Plant Growth Promoting Rhizobacteria produce IAA
 +
*Analogues of phytohormones like α-Naphthaleneacetic acid are usually found in rooting powders. Therefore, if we were to express IAA in bacteria in a controlled fashion we could increase root growth.
 +
*Increased root growth could help maintain top soil.
 +
*Evidence has shown that using IAA on cotton can increase yields.
  
 
===User Reviews===
 
===User Reviews===
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{|width='80%' style='border:1px solid gray'
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<partinfo>BBa_K515100 AddReview 5</partinfo>
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<I>NUDT_CHINA 2015</I>
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|width='60%' valign='top'|
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This part is the only one available with IAAM and IAAH , but unfortunately, we noticed that neither of the CDS of those two enzyme were submitted to the registry (they were only registered as BBa_K515000 and K515001). Thus, several improvements were made based on the part K515100 to provide an available version of IAAM and IAAH.
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====Two pairs of enzymes that can PCR-prep the CDS of IaaM and IaaH====
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For IAAM
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F-Prime: 5’- GGAATTCGCGGCCGCTTCTAGAGATGTTTGGACCGG-3’
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R-Prime: 5’- GCGGCGGACTAGTCTTATTAGTCCCCCAGCG -3’
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For IAAH
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F-Prime: 5’- GGAATTCGCGGCCGCTTCTAGAGATGCGCGAAATG -3’
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R-Prime: 5’- GCGGGCGGCGGACTAGTCTTATTAGCCTTTTAACAC -3’
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====Two new bio-bricks were designed based on this part using the primers above====
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See BBa_K1789000 and BBa_K1789001
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Both of those parts were sent to the registry.
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|};
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<!-- DON'T DELETE --><partinfo>BBa_K515100 EndReviews</partinfo>
 
<!-- DON'T DELETE --><partinfo>BBa_K515100 EndReviews</partinfo>

Latest revision as of 16:27, 18 September 2015

Applications of BBa_K515100

  • The enzymatic reactions of the IAM pathway produce indole-3 acetic acid (IAA) which is an important phytohormone.
  • Many Plant Growth Promoting Rhizobacteria produce IAA
  • Analogues of phytohormones like α-Naphthaleneacetic acid are usually found in rooting powders. Therefore, if we were to express IAA in bacteria in a controlled fashion we could increase root growth.
  • Increased root growth could help maintain top soil.
  • Evidence has shown that using IAA on cotton can increase yields.

User Reviews

UNIQ7a7cae00a419ff59-partinfo-00000001-QINU


•••••

NUDT_CHINA 2015

This part is the only one available with IAAM and IAAH , but unfortunately, we noticed that neither of the CDS of those two enzyme were submitted to the registry (they were only registered as BBa_K515000 and K515001). Thus, several improvements were made based on the part K515100 to provide an available version of IAAM and IAAH.

Two pairs of enzymes that can PCR-prep the CDS of IaaM and IaaH

For IAAM

F-Prime: 5’- GGAATTCGCGGCCGCTTCTAGAGATGTTTGGACCGG-3’

R-Prime: 5’- GCGGCGGACTAGTCTTATTAGTCCCCCAGCG -3’


For IAAH

F-Prime: 5’- GGAATTCGCGGCCGCTTCTAGAGATGCGCGAAATG -3’

R-Prime: 5’- GCGGGCGGCGGACTAGTCTTATTAGCCTTTTAACAC -3’

Two new bio-bricks were designed based on this part using the primers above

See BBa_K1789000 and BBa_K1789001

Both of those parts were sent to the registry.

;


UNIQ7a7cae00a419ff59-partinfo-00000003-QINU