Difference between revisions of "Part:BBa K660200:Design"

 
 
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<partinfo>BBa_K660200 short</partinfo>
 
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===Design Notes===
 
===Design Notes===
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Illegal restriction sites - site directed mutagenesis
 
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===Source===
 
===Source===
  
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Originally isolated from horse sweat.  
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Latherin was originally isolated from horse sweat. It was expressed in <i>E.coli</i> by researchers at University of Glasgow. We received it from them in a plasmid.  We then amplified it up by PCR to add biobrick prefix and suffix.
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Received from researchers
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===References===
 
===References===

Latest revision as of 11:58, 29 September 2011

Latherin (Surfactant Protein)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 200
    Illegal PstI site found at 431
    Illegal PstI site found at 569
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 200
    Illegal PstI site found at 431
    Illegal PstI site found at 569
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 514
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 200
    Illegal PstI site found at 431
    Illegal PstI site found at 569
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 200
    Illegal PstI site found at 431
    Illegal PstI site found at 569
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Source

Latherin was originally isolated from horse sweat. It was expressed in E.coli by researchers at University of Glasgow. We received it from them in a plasmid. We then amplified it up by PCR to add biobrick prefix and suffix.

References