Difference between revisions of "Part:BBa K537015"
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<partinfo>BBa_K537015 short</partinfo> | <partinfo>BBa_K537015 short</partinfo> | ||
| − | + | This composite BioBrick begins with a strong, constitutively active promoter of E.coli (BBa_J23119) followed by an atrazine riboswitch riboswitch (Sinha et al, 2010) which is fused to CheZ and monomeric Red fluorescent reporter protein without stop codons in between. The Freiberg iGEM 2007 BioBrick 3.0 fusion protein assembly was used to construct this BioBrick. The atrazine riboswitch-CheZ fusion was constructed via PCR. This part ends with a standard double terminator transcriptional terminator for E.coli (BBa_B0015). | |
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| + | Note: All scar sites have been excluded in the annotations. There is a scar site between the Promoter:CheZ-mRFP fusion and CheZ-mRFP fusion:Double terminator. The scar sequence is as follows: ACTAGA | ||
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| + | A Freiburg fusion scar site exists between the CheZ and mRFP sequence. The sequence is as follows: ACCGGC | ||
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Latest revision as of 18:15, 19 September 2011
Promoter-Atrazine riboswitch-CheZ-mCherry-Double terminator
This composite BioBrick begins with a strong, constitutively active promoter of E.coli (BBa_J23119) followed by an atrazine riboswitch riboswitch (Sinha et al, 2010) which is fused to CheZ and monomeric Red fluorescent reporter protein without stop codons in between. The Freiberg iGEM 2007 BioBrick 3.0 fusion protein assembly was used to construct this BioBrick. The atrazine riboswitch-CheZ fusion was constructed via PCR. This part ends with a standard double terminator transcriptional terminator for E.coli (BBa_B0015).
Note: All scar sites have been excluded in the annotations. There is a scar site between the Promoter:CheZ-mRFP fusion and CheZ-mRFP fusion:Double terminator. The scar sequence is as follows: ACTAGA
A Freiburg fusion scar site exists between the CheZ and mRFP sequence. The sequence is as follows: ACCGGC
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 44 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1314
Illegal AgeI site found at 1426 - 1000COMPATIBLE WITH RFC[1000]