Difference between revisions of "Part:BBa K537010"

Latest revision as of 12:44, 28 October 2011

Promoter-Theophylline riboswitch 2-Venus-Double terminator

This composite BioBrick begins with a strong, constitutively active promoter of E.coli (BBa_J23119) followed by a theophylline riboswitch (type2) (Lynch and Gallivan NAR 2009) which is fused to the Venus fluorescent reporter protein without a stop codon in between. The theophylline riboswitch2-venus fusion was constructed via 2 rounds of PCR. This part ends with a standard double terminator transcriptional terminator for E.coli (BBa_B0015).

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 730
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

For the pdf version of this datasheet, click here

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 __NOTOC__
 <partinfo>BBa_K537010 short</partinfo>
-Coming soon
+This composite BioBrick begins with a strong, constitutively active promoter of E.coli (BBa_J23119) followed by a theophylline riboswitch (type2) (Lynch and Gallivan NAR 2009) which is fused to the Venus fluorescent reporter protein without a stop codon in between. The theophylline riboswitch2-venus fusion was constructed via 2 rounds of PCR. This part ends with a standard double terminator transcriptional terminator for E.coli (BBa_B0015).
 <!-- Add more about the biology of this part here
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+<html>
+</br>
+</br>
+<p><big>For the pdf version of this datasheet, click <a href="https://static.igem.org/mediawiki/igem.org/f/f5/Datasheet2.pdf">here</a></big></p>
+<center><img src="https://static.igem.org/mediawiki/parts/8/82/Datasheet2.JPG" width="910" vspace="15"></center>
+</html>
 <!-- Uncomment this to enable Functional Parameter display
 ===Functional Parameters===
 <partinfo>BBa_K537010 parameters</partinfo>
 <!-- -->