Difference between revisions of "Part:BBa K590003"
(→‎Usage and Biology)
 
Line 7: Line 7:
 
mamKL is 1336bp in length and the gene numbers are AMB0965 and AMB0966 respectively;  
 
mamKL is 1336bp in length and the gene numbers are AMB0965 and AMB0966 respectively;  
 
it was reported that mamK is required for proper magnetosome chain organization; it is bacterial actin-like cytoskeleton protein required for proper alignment of the magnetosomes in a chain, shown to localize the mamI; mamL is crucial to mangneosome membrane creation, shown to be spread across the cell membrane and sometimes forms lines.
 
it was reported that mamK is required for proper magnetosome chain organization; it is bacterial actin-like cytoskeleton protein required for proper alignment of the magnetosomes in a chain, shown to localize the mamI; mamL is crucial to mangneosome membrane creation, shown to be spread across the cell membrane and sometimes forms lines.
 +
 +
Below is the gel image of mamKL:
 +
[[Image:Igem2011 KL gel.png|center|1kb Ladder (right), mamKL (left)]]
 +
 +
  
 
<!-- -->
 
<!-- -->

Latest revision as of 05:52, 22 September 2011

mamKL, from Magnetospirillum magneticum

This part includes genes mamK and mamL, which were extracted from the genome of Magnetospirillum magneticum strain AMB-1 by [http://2011.igem.org/Team:Washington UW iGEM Team 2011].

Usage and Biology

mamKL is 1336bp in length and the gene numbers are AMB0965 and AMB0966 respectively; it was reported that mamK is required for proper magnetosome chain organization; it is bacterial actin-like cytoskeleton protein required for proper alignment of the magnetosomes in a chain, shown to localize the mamI; mamL is crucial to mangneosome membrane creation, shown to be spread across the cell membrane and sometimes forms lines.

Below is the gel image of mamKL:

1kb Ladder (right), mamKL (left)


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 666
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 666
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 370
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 666
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 666
    Illegal NgoMIV site found at 392
    Illegal NgoMIV site found at 759
    Illegal NgoMIV site found at 906
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1038
    Illegal SapI.rc site found at 1359