Difference between revisions of "Part:BBa K590032"

Latest revision as of 03:58, 29 October 2011

Acyl-ACP Reductase (AAR) - Synechococcus elongatus PCC7942

This part encodes an enzyme that reduces cellular fatty acyl-ACPs, from the bacterial fatty acid biosynthesis pathway, into fatty aldehydes. Together with Aldehyde Decarbonylase (ADC) this part forms the PetroBrick, which generates alkanes from the fatty acid biosynthesis pathway. This part has been codon-optimized for E. coli.

Usage and Biology

When they are expressed together, AAR and ADC produce alkanes from the cell's fatty acid biosynthetic pathway. Alkane production is enhanced when growing expression strains using the growth conditions developed by the [http://2011.igem.org/Team:Washington 2011 University of Washington iGEM Team].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 1060
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K590032 short</partinfo>
-This part encodes an enzyme that reduces cellular fatty acyl-ACPs, from the bacterial fatty acid biosynthesis pathway, into fatty aldehydes.  Use together with [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590031 Aldehyde Decarbonylase] (ADC) to generate alkanes from acyl-ACPs.  This part has been codon-optimized for ''E. coli''.
+This part encodes an enzyme that reduces cellular fatty acyl-ACPs, from the bacterial fatty acid biosynthesis pathway, into fatty aldehydes.  Together with [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590031 Aldehyde Decarbonylase] (ADC) this part forms the [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590025 PetroBrick], which generates alkanes from the fatty acid biosynthesis pathway.  This part has been codon-optimized for ''E. coli''.
 [[Image:Fatty_Acid_Biosynthesis.jpg#file | 250px]]
@@ Line 10: / Line 10: @@
 When they are expressed together, AAR and ADC produce alkanes from the cell's fatty acid biosynthetic pathway.  Alkane production is enhanced when growing expression strains using the growth conditions developed by the [http://2011.igem.org/Team:Washington 2011 University of Washington iGEM Team].
-[[Image:Washinton_AARADCGC-MS.png|left|400px|thumb|GC plot showing that alkanes are only produced when both ADC and AAR are expressed.]]
-[[Image:Washinton_2011_Optimization_Quant.png|right|450px|thumb|GC-MS quantification shows greater alkane production under UW iGEM 2011's "optimized conditions" (see wiki for details)]]