Difference between revisions of "Part:BBa K590032"
 
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<partinfo>BBa_K590032 short</partinfo>
 
<partinfo>BBa_K590032 short</partinfo>
  
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Produces enzyme that reduces long-chain length acyl-ACPs into the corresponding fatty aldehydes.  Works together with [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590031 Aldehyde Decarbonylase] to generate alkanes from acyl-ACPs.   
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This part encodes an enzyme that reduces cellular fatty acyl-ACPs, from the bacterial fatty acid biosynthesis pathway, into fatty aldehydes.  Together with [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590031 Aldehyde Decarbonylase] (ADC) this part forms the [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590025 PetroBrick], which generates alkanes from the fatty acid biosynthesis pathway.  This part has been codon-optimized for ''E. coli''.   
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[[Image:Fatty_Acid_Biosynthesis.jpg#file | 250px]]
  
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
  
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Acyl-ACP Reductase reduces acyl-ACPs into fatty aldehydes.
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When they are expressed together, AAR and ADC produce alkanes from the cell's fatty acid biosynthetic pathway. Alkane production is enhanced when growing expression strains using the growth conditions developed by the [http://2011.igem.org/Team:Washington 2011 University of Washington iGEM Team]. 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K590032 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K590032 SequenceAndFeatures</partinfo>
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Latest revision as of 03:58, 29 October 2011

Acyl-ACP Reductase (AAR) - Synechococcus elongatus PCC7942

This part encodes an enzyme that reduces cellular fatty acyl-ACPs, from the bacterial fatty acid biosynthesis pathway, into fatty aldehydes. Together with Aldehyde Decarbonylase (ADC) this part forms the PetroBrick, which generates alkanes from the fatty acid biosynthesis pathway. This part has been codon-optimized for E. coli.

Fatty Acid Biosynthesis.jpg

Usage and Biology

When they are expressed together, AAR and ADC produce alkanes from the cell's fatty acid biosynthetic pathway. Alkane production is enhanced when growing expression strains using the growth conditions developed by the [http://2011.igem.org/Team:Washington 2011 University of Washington iGEM Team].




Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 1060
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]