Difference between revisions of "Part:BBa K525405:Design"
(→References) |
(→Source) |
||
| Line 24: | Line 24: | ||
** lac-operator from ''E. coli'' | ** lac-operator from ''E. coli'' | ||
| − | * <partinfo>K525401</partinfo> S-layer gene ''sbpA'' synthesized, originated in '' | + | * <partinfo>K525401</partinfo> S-layer gene ''sbpA'' synthesized, originated in ''Lysinibacillus sphaericus'' CCM 2177 |
* <partinfo>J18931</partinfo> yellow fluorescent protein (YFP) mCitrine | * <partinfo>J18931</partinfo> yellow fluorescent protein (YFP) mCitrine | ||
Latest revision as of 18:14, 26 October 2011
Fusion Protein of S-Layer SbpA and mCitrine
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 104
Illegal BglII site found at 221
Illegal XhoI site found at 1996 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 76
Illegal AgeI site found at 3913 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 493
Illegal BsaI.rc site found at 622
Design Notes
- BBa_K525403 fused to BBa_J18931
- BBa_K525403 synthesized, codon optimized and in Freiburg assembly standard (RFC 25) to easily create fusion proteins
- NgoMIV restriction site downstream the start codon to easily fuse an N-terminal domain to the coding sequence
- AgeI restriction site upstream of stop codon to easily fuse a C-terminal domain to the coding sequence
Source
- BBa_K525403 fused to BBa_J18931
- BBa_K525403 synthesized, codon optimized and in Freiburg assembly standard (RFC 25) to easily create fusion proteins
- Promoter: fusion promoter between T7 promoter and lac-operator
- T7 promoter from T7 phage
- lac-operator from E. coli
- BBa_K525401 S-layer gene sbpA synthesized, originated in Lysinibacillus sphaericus CCM 2177
- BBa_J18931 yellow fluorescent protein (YFP) mCitrine
- from parts.igem
- was synthesized before it was sent in
References
Badelt-Lichtblau H, Kainz B, Völlenkle C, Egelseer EM, Sleytr UB, Pum D, Ilk N (2009) Genetic Engineering of the S-Layer Protein SbpA of Lysinibacillus sphaericus CCM 2177 for the Generation of Functionalized Nanoarrays, Bioconjugate Chem [http://pubs.acs.org/doi/abs/10.1021/bc800445r 20(5):895–903].