Difference between revisions of "Part:BBa K590018"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K590018 short</partinfo> | <partinfo>BBa_K590018 short</partinfo> | ||
− | + | This part is made by [http://2011.igem.org/Team:Washington UW iGEM Team 2011]. It includes 5 genes [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590004 mamMN], [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590005 mamO], [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590006 mamPA] that were found essential for magnetosome formation in ''Magnetospirillum magneticum'' strain AMB-1 on a [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590014 pGA3k3 plasmid backbone]. | |
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===Usage and Biology=== | ===Usage and Biology=== | ||
+ | Made by Gibson Cloning method instead of Standard Biobrick method, this part is in a low copy backbone and it belongs to the [http://2011.igem.org/Team:Washington/Magnetosomes/Magnet_Toolkit Magnetosome Toolkit], known as the "middle part of the full assembly". | ||
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+ | [[Image:Igem11MNOPA partregistry.png|500px|center]] | ||
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Latest revision as of 23:21, 20 September 2011
mamMNOPA in vector pGA3K3
This part is made by [http://2011.igem.org/Team:Washington UW iGEM Team 2011]. It includes 5 genes mamMN, mamO, mamPA that were found essential for magnetosome formation in Magnetospirillum magneticum strain AMB-1 on a pGA3k3 plasmid backbone.
Usage and Biology
Made by Gibson Cloning method instead of Standard Biobrick method, this part is in a low copy backbone and it belongs to the [http://2011.igem.org/Team:Washington/Magnetosomes/Magnet_Toolkit Magnetosome Toolkit], known as the "middle part of the full assembly".
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 288
Illegal PstI site found at 74
Illegal PstI site found at 4039
Illegal PstI site found at 4757 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 288
Illegal NheI site found at 1331
Illegal PstI site found at 74
Illegal PstI site found at 4039
Illegal PstI site found at 4757 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 288
Illegal BglII site found at 361
Illegal BamHI site found at 1628
Illegal BamHI site found at 2148
Illegal BamHI site found at 4144 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 288
Illegal PstI site found at 74
Illegal PstI site found at 4039
Illegal PstI site found at 4757 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 288
Illegal PstI site found at 74
Illegal PstI site found at 4039
Illegal PstI site found at 4757
Illegal NgoMIV site found at 3180
Illegal NgoMIV site found at 3553
Illegal NgoMIV site found at 3709
Illegal NgoMIV site found at 3832
Illegal NgoMIV site found at 4627
Illegal NgoMIV site found at 4814
Illegal NgoMIV site found at 5851 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 477
Illegal BsaI.rc site found at 2431
Illegal SapI.rc site found at 56